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Chemical induction of haploid gynogenesis in sterlet Acipenser ruthenus

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F14%3A43886850" target="_blank" >RIV/60076658:12520/14:43886850 - isvavai.cz</a>

  • Result on the web

    <a href="http://www.agriculturejournals.cz/publicFiles/129017.pdf" target="_blank" >http://www.agriculturejournals.cz/publicFiles/129017.pdf</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Chemical induction of haploid gynogenesis in sterlet Acipenser ruthenus

  • Original language description

    Chromosomal manipulations in sturgeons, particularly gynogenesis, are interesting due to the potential to change female ratio in progeny that can be useful for caviar production. The optimization of UV treatment for induction of gynogenesis is complicated due to high and variable optical density of the milt due to differential spermatozoa concentration, and because of sensitivity of spermatozoa's motility apparatus. Therefore in this study we compared chemical methods of sperm treatment as an alternative to short wave-length UV treatment; evaluation considers impact on spermatozoa motility, DNA integrity, and efficiency of DNA inactivation. Dimethyl sulfate (DMS) in concentrations of 2.5-30mM was applied to spermatozoa in order to inactivate DNA. Alsoethidium bromide (EB), psoralen (PS), and 4'-aminomethyl-4,5',8-trimethylpsoralen (AMT) were used to increase sensitivity of spermatozoa's DNA to long wavelength UV-A light (360 nm). CASA analyses of treated sperm showed strong negative e

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    GL - Fishery

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Czech Journal of Animal Science : Živočišná výroba

  • ISSN

    1212-1819

  • e-ISSN

  • Volume of the periodical

    59

  • Issue of the periodical within the volume

    7

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    9

  • Pages from-to

    "310 ?318"

  • UT code for WoS article

  • EID of the result in the Scopus database