FRAP & FLIP: Two sides of the same coin?
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F15%3A43888497" target="_blank" >RIV/60076658:12520/15:43888497 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/15:00443270 RIV/67985807:_____/15:00443270
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
FRAP & FLIP: Two sides of the same coin?
Original language description
The aim of this study is to point out the similarity and differences of data processing based on either FRAP (Fluorescence Recovery After Photobleaching) or FLIP (Fluorescence Loss In Photobleaching) experimental techniques. The core idea, closely related to the sensitivity analysis, is based on discerning between relevant and irrelevant data. Presented mathematical model allows to visualize the mutual relation between the FRAP and FLIP methods. The whole concept resides in the processing of full spatio-temporal data instead of the space averaged time series (FRAP recovery curves). The method theoretically confirms the empirical knowledge, that the mobility of fluorescent molecules can be determined with both FRAP and FLIP methods (using the full data approach). Our analysis, based on the idealized theoretical case study, supports the conclusions of our recent experiments and thus it validates the reliability of our new approach. The presented finding are expected to be reflected into experimental protocol setup as well as we will continue working on the further enhancing the method of parameter identification.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
BA - General mathematics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
ISBN
978-3-319-16479-3
ISSN
0302-9743
e-ISSN
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Number of pages
12
Pages from-to
444-455
Publisher name
Springer Dordrecht
Place of publication
Dordrecht
Event location
Granada; Spain
Event date
Apr 15, 2015
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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