Evaluation of Estrogenic Activity of Wastewater: Comparison Among In Vitro ER alpha Reporter Gene Assay, In Vivo Vitellogenin Induction, and Chemical Analysis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F15%3A43888633" target="_blank" >RIV/60076658:12520/15:43888633 - isvavai.cz</a>
Result on the web
<a href="http://pubs.acs.org/doi/abs/10.1021/acs.est.5b01027" target="_blank" >http://pubs.acs.org/doi/abs/10.1021/acs.est.5b01027</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.est.5b01027" target="_blank" >10.1021/acs.est.5b01027</a>

Result language
angličtina
Original language name
Evaluation of Estrogenic Activity of Wastewater: Comparison Among In Vitro ER alpha Reporter Gene Assay, In Vivo Vitellogenin Induction, and Chemical Analysis
Original language description
The in vitro estrogen receptor (ER) reporter gene assay has long been used to measure estrogenic activity in wastewater. In a previous study, we demonstrated that the assay represents net estrogenic activity in the balance between estrogenic and antiestrogenic activities in waste:water. However, it remained unclear whether the net estrogenic activity measured by the in vitro ER alpha reporter gene assay can predict the in vivo estrogenic effect of wastewater. To determine this, we measured the following: estrogenic and antiestrogenic activities of wastewater and reclaimed water by the in vitro ER alpha reporter gene assay, expression of vitellogenin-1 (vtg1) and choriogenin-H (chgH) in male medaka (Oryzias latipes) by quantitative real-time PCR, and estrone, 17 beta-estradiol, estriol, and 17 alpha-ethynylestradiol concentrations chemically to predict estrogenic activity. The net estrogenic activity measured by the in vitro medaka ER alpha reporter gene assay predicted the in vivo vtg1
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
DJ - Pollution and water control
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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