Novel Technique for Visualizing Primordial Germ Cells in Sturgeons (Acipenser ruthenus, A. gueldenstaedtii, A. baerii, and Huso huso)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F15%3A43888642" target="_blank" >RIV/60076658:12520/15:43888642 - isvavai.cz</a>

Result on the web

<a href="http://www.biolreprod.org/content/93/4/96" target="_blank" >http://www.biolreprod.org/content/93/4/96</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1095/biolreprod.115.128314" target="_blank" >10.1095/biolreprod.115.128314</a>

Result language

angličtina

Original language name

Novel Technique for Visualizing Primordial Germ Cells in Sturgeons (Acipenser ruthenus, A. gueldenstaedtii, A. baerii, and Huso huso)

Original language description

Primordial germ cells (PGCs) are the origin of all germ cells in developing embryos. In the sturgeon embryo, PGCs develop from the vegetal hemisphere, which mainly acts as an extraembryonic source of nutrition. Current methods for studying sturgeon PGCsrequire either killing the fish or using costly and time-consuming histological procedures. Here, we demonstrate that visualization of sterlet (Acipenser ruthenus) PGCs in vivo is feasible by simply labeling the vegetal hemisphere with fluorescein isothiocyanate (FITC)-dextran. We injected FITC-dextrans, with molecular weights varying between 10 000 and 2 000 000, into the vegetal pole of 1- to 4-cell stage embryos. At the neurula to tail-bud developmental stages, FITC-positive PGC-like cells appeared ventrally around the developing tail bud in the experimental group that received a high-molecular-weight FITC-dextran. The highest average number of FITC-positive PGC-like cells was observed in embryos injected with FITC-dextran having a m

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EA - Morphology and cytology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biology of Reproduction

ISSN

0006-3363

e-ISSN

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Volume of the periodical

93

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

7

Pages from-to

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UT code for WoS article

000364587700002

EID of the result in the Scopus database

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