The second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F16%3A43890372" target="_blank" >RIV/60076658:12520/16:43890372 - isvavai.cz</a>

Alternative codes found

RIV/67985904:_____/16:00458511

Result on the web

<a href="http://gsejournal.biomedcentral.com/articles/10.1186/s12711-016-0194-0" target="_blank" >http://gsejournal.biomedcentral.com/articles/10.1186/s12711-016-0194-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12711-016-0194-0" target="_blank" >10.1186/s12711-016-0194-0</a>

Result language

angličtina

Original language name

The second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity

Original language description

Background: One of the five basal actinopterygian lineages, the Chondrostei, including sturgeon, shovelnose, and paddlefish (Order Acipenseriformes) show extraordinary ploidy diversity associated with three rounds of lineage-specific whole-genome duplication, resulting in three levels of ploidy in sturgeon. Recently, incidence of spontaneous polyploidization has been reported among cultured sturgeon and it could have serious negative implications for the economics of sturgeon farming. We report the occurrence of seven spontaneous heptaploid (7n) Siberian sturgeon Acipenser baerii, which is a functional tetraploid species (4n) with similar to 245 chromosomes. Our aims were to assess ploidy level and chromosome number of the analysed specimens and to identify the possible mechanism that underlies the occurrence of spontaneous additional chromosome sets in their genome. Results: Among 150 specimens resulting from the mating of a tetraploid (4n) A. baerii (similar to 245 chromosomes) dam with a hexaploid (6n) A. baerii (similar to 368 chromosomes) sire, 143 displayed a relative DNA content that corresponds to pentaploidy (5n) with an absolute DNA content of 8.98 +/- 0.03 pg DNA per nucleus and nuclear area of 35.3 +/- 4.3 mu m(2) and seven specimens exhibited a relative DNA content that corresponds to heptaploidy (7n), with an absolute DNA content of 15.02 +/- 0.04 pg DNA per nucleus and nuclear area of 48.4 +/- 5.1 mu m(2). Chromosome analyses confirmed a modal number of similar to 437 chromosomes in these heptaploid (7n) individuals. DNA genotyping of eight microsatellite loci followed by parental assignment confirmed spontaneous duplication of the maternal chromosome sets via retention of the second polar body in meiosis II as the mechanism for the formation of this unusual chromosome number and ploidy level in a functional tetraploid A. baerii.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Genetics Selection Evolution

ISSN

0999-193X

e-ISSN

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Volume of the periodical

48

Issue of the periodical within the volume

12

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

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UT code for WoS article

000369815500001

EID of the result in the Scopus database

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