Short-term storage of sterlet Acipenser ruthenus testicular cells at-80 degrees C

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F16%3A43890505" target="_blank" >RIV/60076658:12520/16:43890505 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S001122401630013X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S001122401630013X</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.cryobiol.2016.03.002" target="_blank" >10.1016/j.cryobiol.2016.03.002</a>

Result language
angličtina
Original language name
Short-term storage of sterlet Acipenser ruthenus testicular cells at-80 degrees C
Original language description
The conservation of sturgeons is of critical importance, and optimization of long-term storage is crucial to cell survival. This study aimed to examine the viability rates of several variations of sturgeon testicular cells storage at -80 degrees C for purpose of a short-term storage in a deep freezer or shipment on dried ice. Testes extracted from three immature fish were cut into small pieces, immersed in a cryomedium composed of phosphate buffered saline with 0.5% bovine serum albumin, 50 mM glucose, and 1.5 M ethylene glycol as a cryoprotectant, chilled from 10 to -80 degrees C at a cooling rate of -1 degrees C per min, and stored under varying conditions. Our results revealed a significant effect of storage conditions on the number of living and dead cells (p > 0.05). Samples that were stored for 7 days at -80 degrees C showed a considerable decline in terms of cell viability compared to samples stored for 2 days storage at -80 degrees C or in LN. This result indicated that testicular cells can be stored at -80 degrees C and/or on dry ice, for 2 days with minimum loss of viability.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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