Preservation of female genetic resources of common carp through oogonial stem cell manipulation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F19%3A43899051" target="_blank" >RIV/60076658:12520/19:43899051 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0011224018306084?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0011224018306084?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cryobiol.2019.01.016" target="_blank" >10.1016/j.cryobiol.2019.01.016</a>

Result language

angličtina

Original language name

Preservation of female genetic resources of common carp through oogonial stem cell manipulation

Original language description

Several experiments were conducted in order to develop an optimal protocol for slow-rate freezing ( -1 degrees C/min) and short-term storage ( - 80 or 4 degrees C) of common carp ovarian tissue fragments with an emphasis on oogonial stem cells (OSCs). Dimethyl sulfoxide (Me2SO) with concentration of 1.5 M was identified as the best cryoprotectant in comparison to propylene glycol and methanol. When comparing supplementation of sugars (glucose, trehalose, sucrose) in different concentrations (0.1, 0.3, 0.5 M), glucose and trehalose in 0.3 M were identified as optimal. Short-term storage options for ovarian tissue pieces at -80 degrees C and 4 degrees C were tested as alternatives to cryopreservation and storage in liquid nitrogen. The presence of OSCs was confirmed by immunocytochemistry and viability after storage was determined by the trypan blue exclusion test. This study identified the optimal protocol for OSC cryopreservation using slow rate freezing resulting in similar to 65% viability. The frozen/thawed OSCs were labelled by PKH-26 and transplanted into goldfish recipients. The success of the transplantation was confirmed by presence of fluorescent cells in the recipient gonad and later on by RT-PCR with carp dnd1 specific primers. The results of this study can facilitate long-term preservation of common carp germplasm which can be recovered in a surrogate recipient through interspecific germ cell transplantation.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40103 - Fishery

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cryobiology

ISSN

0011-2240

e-ISSN

—

Volume of the periodical

87

Issue of the periodical within the volume

neuveden

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

78-85

UT code for WoS article

000465154600011

EID of the result in the Scopus database

2-s2.0-85061014992