Cryopreservation of Carp (Cyprinus carpio L.) Sperm: Impact of Seeding and Freezing Rates on Post-Thaw Outputs
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F17%3A43895335" target="_blank" >RIV/60076658:12520/17:43895335 - isvavai.cz</a>
Result on the web
<a href="http://online.liebertpub.com/doi/10.1089/bio.2016.0065" target="_blank" >http://online.liebertpub.com/doi/10.1089/bio.2016.0065</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1089/bio.2016.0065" target="_blank" >10.1089/bio.2016.0065</a>
Alternative languages
Result language
angličtina
Original language name
Cryopreservation of Carp (Cyprinus carpio L.) Sperm: Impact of Seeding and Freezing Rates on Post-Thaw Outputs
Original language description
In the present study, we examined various freezing protocols, effects of controlled seeding, and changes in cooling rate and determined the endpoint (temperature at which sample could be plugged into liquid nitrogen (LN) without visible effect on survival rate after thawing) to reveal the relative importance of each different stage of cooling on freezing success during cryobanking of carp sperm. Sperm samples from different individual carp males were frozen in 0.5 mL straws by conventional freezing. Cooling rates were determined by monitoring the sample's internal temperature. We compared four freezing protocols, which involved placing sperm samples at various levels (1, 3, 6, and 9cm) above the LN surface (corresponding to -190 degrees C, -150 degrees C, -110 degrees C, and -70 degrees C, respectively) for 20 minutes followed by transferring the samples into LN. Freezing at 3cm above the LN surface resulted in the highest motility (33%+/- 8%) and velocity (118 +/- 9 mu/s) of spermatozoa after thawing and diluting in swimming medium. We determined that -90 degrees C is an optimal temperature at which immersing the samples in LN does not affect sperm motility after thawing and shorten the process of freezing for around three times. Motility of spermatozoa cryopreserved with or without a seeding procedure was not significantly different after thawing. Therefore, we hypothesize that supercooling the sample during the conventional freezing procedure is not the main damaging factor during carp spermatozoa cryopreservation. However, the cooling rate itself is important, because it determines the ability of the sperm to dehydrate and survive cryopreservation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40103 - Fishery
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biopreservation and Biobanking
ISSN
1947-5535
e-ISSN
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Volume of the periodical
15
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
234-240
UT code for WoS article
000402710100010
EID of the result in the Scopus database
2-s2.0-85020213092