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The Timing and Characterization of Maternal to Zygote Transition and Mid-Blastula Transition in Sterlet Acipenser ruthenus and A. ruthenus x Acipenser gueldenstaedtii Hybrid

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F19%3A43899034" target="_blank" >RIV/60076658:12520/19:43899034 - isvavai.cz</a>

  • Alternative codes found

    RIV/67985904:_____/19:00503711 RIV/61988987:17310/19:A21025N6

  • Result on the web

    <a href="http://trjfas.org/uploads/pdf_1328.pdf" target="_blank" >http://trjfas.org/uploads/pdf_1328.pdf</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.4194/1303-2712-v19_2_09" target="_blank" >10.4194/1303-2712-v19_2_09</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    The Timing and Characterization of Maternal to Zygote Transition and Mid-Blastula Transition in Sterlet Acipenser ruthenus and A. ruthenus x Acipenser gueldenstaedtii Hybrid

  • Original language description

    Detailed knowledge of early embryo development is a prerequisite for implementation of reproductive biotechnologies. We described the timing of mid-blastula transition in the sturgeon Acipenser ruthenus and hybrid of A. ruthenus and Acipenser gueldenstaedtii. To identify mitotic changes during cell division, the nuclei of embryo blastomeres were sampled every 30 min from 4 to 25 h post-fertilization and stained with 5 mg/ml 4&apos;-6-diaminido-2phenylindole. We observed embryos of hybrids to lose synchrony after the eighth cell division, one cycle earlier than in purebred sterlet embryos. To reveal the timing of zygotic gene activation, developing embryos were dechorionated and injected with 10, 50, 100, 250, or 500 mu g/ml a-Amanitin. Termination of development in embryos was recorded throughout the period from the 1000 cell stage until gastrulation, showing the process of transition from maternal to zygotic gene transcription in sterlet to be of extended duration and not directly linked to mid-blastula transition.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    40103 - Fishery

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Turkish Journal of Fisheries and Aquatic Sciences

  • ISSN

    1303-2712

  • e-ISSN

  • Volume of the periodical

    19

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    TR - TURKEY

  • Number of pages

    8

  • Pages from-to

    77-84

  • UT code for WoS article

    000443957500009

  • EID of the result in the Scopus database

    2-s2.0-85053340233