The Timing and Characterization of Maternal to Zygote Transition and Mid-Blastula Transition in Sterlet Acipenser ruthenus and A. ruthenus x Acipenser gueldenstaedtii Hybrid

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F19%3A43899034" target="_blank" >RIV/60076658:12520/19:43899034 - isvavai.cz</a>
Alternative codes found
RIV/67985904:_____/19:00503711 RIV/61988987:17310/19:A21025N6
Result on the web
<a href="http://trjfas.org/uploads/pdf_1328.pdf" target="_blank" >http://trjfas.org/uploads/pdf_1328.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4194/1303-2712-v19_2_09" target="_blank" >10.4194/1303-2712-v19_2_09</a>

Result language
angličtina
Original language name
The Timing and Characterization of Maternal to Zygote Transition and Mid-Blastula Transition in Sterlet Acipenser ruthenus and A. ruthenus x Acipenser gueldenstaedtii Hybrid
Original language description
Detailed knowledge of early embryo development is a prerequisite for implementation of reproductive biotechnologies. We described the timing of mid-blastula transition in the sturgeon Acipenser ruthenus and hybrid of A. ruthenus and Acipenser gueldenstaedtii. To identify mitotic changes during cell division, the nuclei of embryo blastomeres were sampled every 30 min from 4 to 25 h post-fertilization and stained with 5 mg/ml 4'-6-diaminido-2phenylindole. We observed embryos of hybrids to lose synchrony after the eighth cell division, one cycle earlier than in purebred sterlet embryos. To reveal the timing of zygotic gene activation, developing embryos were dechorionated and injected with 10, 50, 100, 250, or 500 mu g/ml a-Amanitin. Termination of development in embryos was recorded throughout the period from the 1000 cell stage until gastrulation, showing the process of transition from maternal to zygotic gene transcription in sterlet to be of extended duration and not directly linked to mid-blastula transition.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40103 - Fishery

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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