The Timing and Characterization of Maternal to Zygote Transition and Mid-Blastula Transition in Sterlet Acipenser ruthenus and A. ruthenus x Acipenser gueldenstaedtii Hybrid
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F19%3AA21025N6" target="_blank" >RIV/61988987:17310/19:A21025N6 - isvavai.cz</a>
Alternative codes found
RIV/67985904:_____/19:00503711 RIV/60076658:12520/19:43899034
Result on the web
<a href="http://www.trjfas.org/uploads/pdf_1328.pdf" target="_blank" >http://www.trjfas.org/uploads/pdf_1328.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4194/1303-2712-v19_2_09" target="_blank" >10.4194/1303-2712-v19_2_09</a>
Alternative languages
Result language
angličtina
Original language name
The Timing and Characterization of Maternal to Zygote Transition and Mid-Blastula Transition in Sterlet Acipenser ruthenus and A. ruthenus x Acipenser gueldenstaedtii Hybrid
Original language description
Detailed knowledge of early embryo development is a prerequisite for implementation of reproductive biotechnologies. We described the timing of mid-blastula transition in the sturgeon Acipenser ruthenus and hybrid of A. ruthenus and Acipenser gueldenstaedtii. To identify mitotic changes during cell division, the nuclei of embryo blastomeres were sampled every 30 min from 4 to 25 h post-fertilization and stained with 5 mg/ml 4'-6-diaminido-2phenylindole. We observed embryos of hybrids to lose synchrony after the eighth cell division, one cycle earlier than in purebred sterlet embryos. To reveal the timing of zygotic gene activation, developing embryos were dechorionated and injected with 10, 50, 100, 250, or 500 mu g/ml a-Amanitin. Termination of development in embryos was recorded throughout the period from the 1000 cell stage until gastrulation, showing the process of transition from maternal to zygotic gene transcription in sterlet to be of extended duration and not directly linked to mid-blastula transition.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10605 - Developmental biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Turkish Journal of Fisheries and Aquatic Sciences
ISSN
1303-2712
e-ISSN
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Volume of the periodical
19
Issue of the periodical within the volume
2
Country of publishing house
TR - TURKEY
Number of pages
8
Pages from-to
77-84
UT code for WoS article
000443957500009
EID of the result in the Scopus database
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