Production and use of triploid zebrafish for surrogate reproduction
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F19%3A43899474" target="_blank" >RIV/60076658:12520/19:43899474 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0093691X19303565" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0093691X19303565</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.theriogenology.2019.08.016" target="_blank" >10.1016/j.theriogenology.2019.08.016</a>
Alternative languages
Result language
angličtina
Original language name
Production and use of triploid zebrafish for surrogate reproduction
Original language description
We report for the first time, a comparison of two approaches for artificially induced triploidy in zebrafish (Danio rerio) using cold shock and heat shock treatments. Of the two methods, heat shock treatment proved more effective with a triploid production rate of 100% in particular females. Subsequently, triploid zebrafish larvae were used as recipients for intraperitoneal transplantation of ovarian and testicular cells originating from vas:EGFP strain in order to verify their suitability for surrogate reproduction. Production of donor-derived sperm was achieved in 23% of testicular cell recipients and 16% of ovarian cell recipients, indicating the suitability of triploids as surrogate hosts for germ cell transplantation. Success of the transplantation was confirmed by positive GFP signal detected in gonads of dissected fish and stripped sperm. Germline transmission was confirmed by fertilization tests followed by PCR analysis of embryos with GFP specific primers. Reproductive success of germline chimera triploids evaluated as fertilization rate and progeny development was comparable to control groups. (C) 2019 Elsevier Inc. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10604 - Reproductive biology (medical aspects to be 3)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Theriogenology
ISSN
0093-691X
e-ISSN
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Volume of the periodical
140
Issue of the periodical within the volume
neuveden
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
33-43
UT code for WoS article
000492488000005
EID of the result in the Scopus database
2-s2.0-85070629288