Interleukin-1 beta induces autophagy of mouse preimplantation embryos and improves blastocyst quality

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43900802" target="_blank" >RIV/60076658:12520/20:43900802 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1002/jcb.29345" target="_blank" >https://doi.org/10.1002/jcb.29345</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jcb.29345" target="_blank" >10.1002/jcb.29345</a>

Result language

angličtina

Original language name

Interleukin-1 beta induces autophagy of mouse preimplantation embryos and improves blastocyst quality

Original language description

Autophagy is one of the basic cellular mechanism during preimplantation development of mammalian embryos, and it plays crucial role in several physiological processes. It is induced by interleukin (IL)-1 beta in mammalian cells. Our present study shows that IL-1 beta is important for autophagy activation in embryo development. Our in vitro culture system analysis shows effect of IL-1 beta in medium on the development of mouse embryos and it was found to be concentration dependent. A preimplantation embryo culture using medium containing IL-1 beta did not improve cleavage and blastocyst development rates of mouse embryos; however, blastocyst quality was significantly improved by increasing total cell number, especially in supplementary 20 ng/mL IL-1 beta. Furthermore, autophagy activation mainly occurs in 2 to 4 cell embryo and blastocyst, 20 ng/mL IL-1 beta into culture medium can effectively enhance levels of messenger RNA and protein of autophagy-related-factors in 2 to 4 cell embryos and blastocyst, while these factors reduce in VGX-1027 (IL-1 beta inhibitor) groups that also reduce the quality of blastocyst. Effects of IL-1 beta on the development of embryo reduced in 20 ng/mL IL-1 beta supplemented group when 5 mM 3-methyladenine (3-MA) was also added, which used to inhibit autophagy activation in endogenous PtdIns3Ks signal pathway. Our current results show that exogenous IL-1 beta can effectively induce autophagy in mouse embryos at stages of 2 to 8 cell and blastocyst, that also help to improve the quality of blastocyst.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10604 - Reproductive biology (medical aspects to be 3)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Cellular Biochemistry

ISSN

0730-2312

e-ISSN

—

Volume of the periodical

121

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

1087-1100

UT code for WoS article

000483802600001

EID of the result in the Scopus database

2-s2.0-85071632348