Application of hydrostatic pressure shock for retention of the second polar body in sterlet (Acipenser ruthenus)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43900813" target="_blank" >RIV/60076658:12520/20:43900813 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.aquaculture.2020.734947" target="_blank" >https://doi.org/10.1016/j.aquaculture.2020.734947</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2020.734947" target="_blank" >10.1016/j.aquaculture.2020.734947</a>

Result language

angličtina

Original language name

Application of hydrostatic pressure shock for retention of the second polar body in sterlet (Acipenser ruthenus)

Original language description

Induced retention of the second polar body (SPBR) is used for production of triploid fish and/or for restoration of diploidy during induced meiotic gynogenesis (MeiG). Using sterlet, Acipenser ruthenus as model species of sturgeon, a protocol was developed for SPBR with hydrostatic pressure shock, commonly found more considerate for treated eggs of teleosts, instead of heat shock conventionally used hitherto. In the first trial, hydrostatic pressure shock of 55 MPa for 3 min duration applied to eggs 18 min post gamete activation gave the highest fertilization rate (62.14 ± 8.90%) and hatching rate (24.59 ± 8.35%) both on Petri dishes and after pilot testing in Zuger jars (78.55%; 45.75%, respectively). Flow cytometric analysis of relative DNA content confirmed 100% triploidy in all sampled prelarvae from shocked eggs. In the second trial comparing the effectiveness of 55 MPa hydrostatic pressure shock for SPBR with conventional 35 °C heat shock for 3 min duration in order to induce triploidy and/or MeiG, pressure – induced triploids exhibited fertilization rate 62.14 ± 8.90% significantly lower than the control group (79.17 ± 7.64%) but indifferent from that in heat -shocked triploids (60.08 ± 4.65%). In MeiG groups, rediploidization with pressure shock or with heat shock did not reveal significant differences in fertilization rate, triploidization with pressure shock provided significantly higher value (46.54 ± 7.47%) than that obtained with heat shock (13.93 ± 3.46%), while similar to the control group (52.71 ± 1.9%). Hatching rates in both MeiG groups did not differ significantly each other but from values in the control group and in the pressure-induced triploids. Flow cytometric analysis of relative DNA content confirmed 100% and 80% triploidy in prelarvae sampled from the pressure- and heat shocked triploids, respectively. Among the MeiG fish, 26 and 25 analyzed prelarvae out of 30 from each the pressure- and heat shock-rediploidized groups were diploid and the remaining prelarvae were haploid. This experiment confirmed the suitability of 55 MPa hydrostatic pressure shock for SPBR in sturgeons. © 2020 Elsevier B.V.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40103 - Fishery

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Aquaculture

ISSN

0044-8486

e-ISSN

—

Volume of the periodical

520

Issue of the periodical within the volume

520

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

—

UT code for WoS article

000515498200046

EID of the result in the Scopus database

2-s2.0-85077923087