Molecular Characterization of PGC-1 beta (PPAR Gamma Coactivator 1 beta) and its Roles in Mitochondrial Biogenesis in Blunt Snout Bream (Megalobrama amblycephala)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43900894" target="_blank" >RIV/60076658:12520/20:43900894 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.3390/ijms21061935" target="_blank" >https://doi.org/10.3390/ijms21061935</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms21061935" target="_blank" >10.3390/ijms21061935</a>

Result language

angličtina

Original language name

Molecular Characterization of PGC-1 beta (PPAR Gamma Coactivator 1 beta) and its Roles in Mitochondrial Biogenesis in Blunt Snout Bream (Megalobrama amblycephala)

Original language description

This study aimed at achieving the molecular characterization of peroxisome proliferator-activated receptor-gamma coactivator 1 beta (PGC-1 beta) and exploring its modulatory roles in mitochondria biogenesis in blunt snout bream (Megalobrama amblycephala). A full-length cDNA of PGC-1 beta was cloned from liver which covered 3110 bp encoding 859 amino acids. The conserved motifs of PGC-1 beta family proteins were gained by MEME software, and the phylogenetic analyses showed motif loss and rearrangement of PGC-1 beta in fish. The function of PGC-1 beta was evaluated through overexpression and knockdown of PGC-1 beta in primary hepatocytes of blunt snout bream. We observed overexpression of PGC-1 beta along with enhanced mitochondrial transcription factor A (TFAM) expression and mtDNA copies in hepatocytes, and its knockdown led to slightly reduced NRF1 expression. However, knockdown of PGC-1 beta did not significantly influence TFAM expression or mtDNA copies. The alterations in mitochondria biogenesis were assessed following high-fat intake, and the results showed that it induces downregulation of PGC-1 beta. Furthermore, significant decreases in mitochondrial respiratory chain activities and mitochondria biogenesis were observed by high-fat intake. Our findings demonstrated that overexpression of PGC-1 beta induces the enhancement of TFAM expression and mtDNA amount but not NRF-1. Therefore, it could be concluded that PGC-1 beta is involved in mitochondrial biogenesis in blunt snout bream but not through PGC-1 beta/NRF-1 pathway.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40103 - Fishery

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

1422-0067

Volume of the periodical

21

Issue of the periodical within the volume

6

Country of publishing house

CH - SWITZERLAND

Number of pages

13

Pages from-to

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UT code for WoS article

000529890200031

EID of the result in the Scopus database

2-s2.0-85081615701