Standardization of egg activation and fertilization in sterlet (Acipenser ruthenus)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43900933" target="_blank" >RIV/60076658:12520/20:43900933 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.aqrep.2020.100381" target="_blank" >https://doi.org/10.1016/j.aqrep.2020.100381</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aqrep.2020.100381" target="_blank" >10.1016/j.aqrep.2020.100381</a>

Result language

angličtina

Original language name

Standardization of egg activation and fertilization in sterlet (Acipenser ruthenus)

Original language description

This study was conducted to determine the minimum number of spermatozoa and optimal volume of activation water for effective fertilization of sterlet Acipenser nnhenus eggs. It was also to determine the minimum concentration of spermatozoa per ml of activation water during artificial propagation, when sperm with good motility and velocity (95 % motility and 150 mu m s(-1) curvilinear velocity) were used for fertilization. In three experiments, eggs (similar to 50) from three sterlet were fertilized by pooled sperm from four males in four replicates and incubated at 17 degrees C. The success of fertilization was evaluated at neurulation and hatching. Sterlet eggs were fertilized in proportion to the concentration of spermatozoa per ml in activation water. A total of 50,000 spermatozoa in 8 ml or 32 ml of activation water (concentrations of 6,250 and 1,563 spermatozoa per ml of activation water, respectively) was sufficient for successful fertilization based on neurulation and hatching rates. Neurulation and hatching gradually decreased with a decrease of the spermatozoa egg ratio from 6,250 to 114. A ratio 1 divided by 4 between eggs and water was insufficient to provide adequate fertilization. Highest neurulation and hatching ratios were reported when the egg divided by water ratio reached 1 divided by 15, which is recommended for practical usage. Our results therefore showed that a very low concentration of 100 - 1,300 spermatozoa per ml with around 95 % motility and curvilinear velocity over 150 mu m s(-1) in activation water can provide effective fertilization. In studies of fishes with external fertilization, the volume of activation water during fertilization, total number of spermatozoa and eggs and/or concentration of sperm in activation water should be defined.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40103 - Fishery

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Aquaculture Reports

ISSN

2352-5134

e-ISSN

—

Volume of the periodical

17

Issue of the periodical within the volume

neuveden

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

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UT code for WoS article

000544610700006

EID of the result in the Scopus database

2-s2.0-85085327351