Energy pathways associated with sustained spermatozoon motility in the endangered Siberian sturgeonAcipenser baerii
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43901019" target="_blank" >RIV/60076658:12520/20:43901019 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1111/jfb.14382" target="_blank" >https://doi.org/10.1111/jfb.14382</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jfb.14382" target="_blank" >10.1111/jfb.14382</a>
Alternative languages
Result language
angličtina
Original language name
Energy pathways associated with sustained spermatozoon motility in the endangered Siberian sturgeonAcipenser baerii
Original language description
Sturgeon spermatozoa are unique for their sustained motility. We investigated the relative importance of bioenergetic pathways in the energy supply of Siberian sturgeonAcipenser baeriispermatozoa during motile and immotile states. Spermatozoon motility and oxygen consumption rate (OCR) were analysed following exposure to inhibitors of oxidative phosphorylation (sodium azide, NaN3), glycolysis (2-deoxy-D-glucose, DOG) and beta-oxidation of fatty acids (sodium fluoride, NaF), and to an uncoupler of oxidative phosphorylation (carbonyl cyanidem-chlorophenyl hydrazine, CCCP). No significant difference in curvilinear velocity was observed after addition of these reagents to activation medium (AM) or nonactivation medium (NAM) for incubation. Incubation of spermatozoa in NAM containing CCCP or NaN(3)resulted in significantly decreased motility duration compared to controls. The OCR of sturgeon spermatozoa in AM (11.9 +/- 1.4 nmol O(2)min(-1)(10(9) spz)(-1)) was significantly higher than in NAM (8.2 +/- 1.5 nmol O(2)min(-1)(10(9) spz)(-1)). The OCR significantly declined with addition of NaN(3)to AM and NAM. No significant difference in motility parameters or OCR was observed with NaF or DOG. These results suggest active oxidative phosphorylation in both immotile and motile spermatozoa. Nevertheless, mitochondrial respiration occurring during motility is not sufficient to meet the high energy demands, and the energy required for sustained motility of Siberian sturgeon spermatozoa is derived from adenosine triphosphate accumulated during the quiescent state.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40103 - Fishery
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Fish Biology
ISSN
0022-1112
e-ISSN
1095-8649
Volume of the periodical
97
Issue of the periodical within the volume
2
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
435-443
UT code for WoS article
000541450100001
EID of the result in the Scopus database
2-s2.0-85087170589