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Energy pathways associated with sustained spermatozoon motility in the endangered Siberian sturgeonAcipenser baerii

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43901019" target="_blank" >RIV/60076658:12520/20:43901019 - isvavai.cz</a>

  • Result on the web

    <a href="https://doi.org/10.1111/jfb.14382" target="_blank" >https://doi.org/10.1111/jfb.14382</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1111/jfb.14382" target="_blank" >10.1111/jfb.14382</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Energy pathways associated with sustained spermatozoon motility in the endangered Siberian sturgeonAcipenser baerii

  • Original language description

    Sturgeon spermatozoa are unique for their sustained motility. We investigated the relative importance of bioenergetic pathways in the energy supply of Siberian sturgeonAcipenser baeriispermatozoa during motile and immotile states. Spermatozoon motility and oxygen consumption rate (OCR) were analysed following exposure to inhibitors of oxidative phosphorylation (sodium azide, NaN3), glycolysis (2-deoxy-D-glucose, DOG) and beta-oxidation of fatty acids (sodium fluoride, NaF), and to an uncoupler of oxidative phosphorylation (carbonyl cyanidem-chlorophenyl hydrazine, CCCP). No significant difference in curvilinear velocity was observed after addition of these reagents to activation medium (AM) or nonactivation medium (NAM) for incubation. Incubation of spermatozoa in NAM containing CCCP or NaN(3)resulted in significantly decreased motility duration compared to controls. The OCR of sturgeon spermatozoa in AM (11.9 +/- 1.4 nmol O(2)min(-1)(10(9) spz)(-1)) was significantly higher than in NAM (8.2 +/- 1.5 nmol O(2)min(-1)(10(9) spz)(-1)). The OCR significantly declined with addition of NaN(3)to AM and NAM. No significant difference in motility parameters or OCR was observed with NaF or DOG. These results suggest active oxidative phosphorylation in both immotile and motile spermatozoa. Nevertheless, mitochondrial respiration occurring during motility is not sufficient to meet the high energy demands, and the energy required for sustained motility of Siberian sturgeon spermatozoa is derived from adenosine triphosphate accumulated during the quiescent state.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    40103 - Fishery

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Fish Biology

  • ISSN

    0022-1112

  • e-ISSN

    1095-8649

  • Volume of the periodical

    97

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    9

  • Pages from-to

    435-443

  • UT code for WoS article

    000541450100001

  • EID of the result in the Scopus database

    2-s2.0-85087170589