Quantification of adenosine triphosphate, adenosine diphosphate, and creatine phosphate in sterlet Acipenser ruthenus spermatozoa during maturation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F15%3A43888847" target="_blank" >RIV/60076658:12520/15:43888847 - isvavai.cz</a>
Result on the web
<a href="https://dl.sciencesocieties.org/publications/jas/abstracts/93/11/5214" target="_blank" >https://dl.sciencesocieties.org/publications/jas/abstracts/93/11/5214</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2527/jas2015-9144" target="_blank" >10.2527/jas2015-9144</a>

Result language
angličtina
Original language name
Quantification of adenosine triphosphate, adenosine diphosphate, and creatine phosphate in sterlet Acipenser ruthenus spermatozoa during maturation
Original language description
Sturgeon spermatozoa maturation during their passage through the kidney is a prerequisite for initiation of motility. Samples of sterlet (Acipenser ruthenus) testicular sperm (TS) were matured in vitro by incubation in seminal fluid (SF) or in SF supplemented with carbonyl cyanide m-chlorophenyl hydrazone (CCCP; a respiration uncoupling agent). Sperm was diluted in activation medium (AM) containing 10 mM Tris-HCl buffer (pH 8.5) and 0.25% Pluronic, and spermatozoon motility was assessed. Samples were taken and fixed in 3 M perchloric acid at 3 points in the incubation process. Quantification of ATP, ADP, and creatine phosphate (CrP) was conducted using liquid chromatography/high-resolution mass spectrometry. We observed a significant decrease in CrP during artificial maturation of TS in SF. In contrast, ATP and ADP were not significantly affected. Addition of CCCP to SF halted maturation and led to significantly lower CrP whereas ADP significantly increased and ATP was unaffected. Dilu
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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