First identification of dopamine receptors in pikeperch, Sander lucioperca, during the pre-ovulatory period

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43901152" target="_blank" >RIV/60076658:12520/20:43901152 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.cbd.2020.100747" target="_blank" >https://doi.org/10.1016/j.cbd.2020.100747</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cbd.2020.100747" target="_blank" >10.1016/j.cbd.2020.100747</a>

Result language

angličtina

Original language name

First identification of dopamine receptors in pikeperch, Sander lucioperca, during the pre-ovulatory period

Original language description

Dopamine (DA) is a ubiquitous neurotransmitter exerting a range of pleiotropic actions through two DA receptor families, the D1 and the D2. To date in vertebrates, a maximum of four receptor subtypes have been identified within the D1 family, D-1 (former D-1A), D-5 (former D-1B), D-6 (former D-1C and D-1D) and D-7 (former D-1E), while the D2 family encloses five subtypes, D-2, D-3, D-4, D-8 (former D-2like or D-2l) and D-9 (former D4-related sequence or D4-rs). In teleosts, no study has investigated in parallel all the DA receptors to identify and localize the whole receptor repertoire from both families. In pikeperch, Sander lucioperca, a species of interest for aquaculture development, the existence, number and location of the DA receptors are totally unknown. To address these questions, RNA-seq with de novo transcriptome reconstruction, functional annotation and phylogenetic analysis were performed to characterize the transcript repertoire of DA receptors in the brain of female pikeperch at the pre-ovulatory period. Ten different cDNA were identified and showed to belong to the D-1 family: two D-1, one D-5a, one D-6a and one D-6b and to the D2 family: two spliced variants of D-2, one D-3, one D-8 and one D-9. Unlike zebrafish, the subtypes D-4 and D-7 have not yet been isolated in pikeperch. As expected D-1, D-3, D-8 and D-9 are mostly expressed in brain parts except for the cerebellum (D-1 and D-3). The inter-species differences in the number of DA receptors and the inter-organ differences in the gene expression of all receptors support the complexity of the dopaminergic actions in vertebrate.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10604 - Reproductive biology (medical aspects to be 3)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Comparative Biochemistry and Physiology D-Genomics &amp; Proteomics

ISSN

1744-117X

e-ISSN

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Volume of the periodical

36

Issue of the periodical within the volume

neuveden

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

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UT code for WoS article

000591917000008

EID of the result in the Scopus database

2-s2.0-85091766161