Optimization of short-term storage of pikeperch semen: an applicable approach

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43902525" target="_blank" >RIV/60076658:12520/21:43902525 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.animal.2020.100001" target="_blank" >https://doi.org/10.1016/j.animal.2020.100001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.animal.2020.100001" target="_blank" >10.1016/j.animal.2020.100001</a>

Result language

angličtina

Original language name

Optimization of short-term storage of pikeperch semen: an applicable approach

Original language description

Contamination of semen with urine and asynchronous maturation of males and females are main obstacles in artificial reproduction of pikeperch Sander lucioperca. The objective of this study was to overcome these obstacles using optimization of a procedure for short-term storage of pikeperch semen at 4 degrees C using two immobilizing media (IM): (a) IM1, 180 mM NaCl, 2.68 mM KCl, 1.36 mM CaCl2 center dot 2H(2)O and 2.38 mM NaHCO3, 343 mOsm/kg; and (b) IM2, 200 mM NaCl, 2.68 mM Ka, 1.36 mM CaCl2 center dot 2H(2)O and 2.38 mM NaHCO3, 381 mOsm/kg. Undiluted sperm was used as the control. At 6 h poststorage, there were no substantial changes in spermatozoa motility and velocity at 30 s postactivation in all groups. Over 48 h of storage, the highest spermatozoa motility and velocity were obtained in sperm diluted in IM2 compared to the other groups. IM2 could maintain a significantly higher ATP content of diluted sperm than IM1 and undiluted treatment fort days. Similarly, the highest values of eyeing and hatching rates were observed in sperm diluted in IM2 compared to sperm in the other studied groups. It can be concluded that the obtained result is a novel and applicable approach to maintain semen quality of pikeperch during short-term storage, suggesting IM2 as a promising medium for short-term storage. The present study also opens possibilities for ensuring a reliable source of semen as a convenient approach for increasing genetic diversity in hatcheries. (C) 2020 Published by Elsevier Inc. on behalf of The Animal Consortium.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40103 - Fishery

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Animal

ISSN

1751-7311

e-ISSN

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Volume of the periodical

15

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

6

Pages from-to

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UT code for WoS article

000626637900001

EID of the result in the Scopus database

2-s2.0-85100670584