Effect of appropriate extenders to maintain sperm functionality during short-term storage of sterlet (Acipenser ruthenus) sperm with fertilization assay under hatchery conditions

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F25%3A43909638" target="_blank" >RIV/60076658:12520/25:43909638 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1007/s10695-024-01413-7" target="_blank" >https://doi.org/10.1007/s10695-024-01413-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10695-024-01413-7" target="_blank" >10.1007/s10695-024-01413-7</a>

Result language

angličtina

Original language name

Effect of appropriate extenders to maintain sperm functionality during short-term storage of sterlet (Acipenser ruthenus) sperm with fertilization assay under hatchery conditions

Original language description

Short-term storage and management of sperm in vitro is an easy and economical process in which suitable extenders can be utilized to extend the storage period and prevent sperm function impairment. Therefore, the current study aimed to evaluate the effect of suitable extenders during the short-term storage of sterlet sperm and determine their fertilizing capacity and hatching success. Three extenders containing a composition of 16, 20, and 24 mM NaCl, 1 mM KCl, 0.1 mM CaCl2, 10 mM Tris, pH 8.0 with osmolarity of 46, 55, and 62 mOsm/kg, were used to dilute the sperm of four sexually mature sterlet males (n = 4). Using a CASA system, the motility and velocity of undiluted and diluted sperm with extenders (E1 - E3) were assessed over 6 days at 0-2 degrees C. The short-term stored diluted sperm was then used in the fertilization and hatching assay, and undiluted fresh and stored sperm was used as a control. A two-way factorial analysis of variance (ANOVA) model confirmed significant effects on sperm motility, curvilinear velocity (VCL), and straight-line velocity (VSL) (P &lt; 0.001), as well as their interaction with the extender. The model was decomposed into a one-way ANOVA to examine the impacts of extenders and storage time. With increasing storage periods, the sperm motility and velocity gradually decreased for diluted sperm with three extenders (E1-E3) but sharply decreased for undiluted sperm (Control). The motility of undiluted sperm was found 3.77 +/- 4.09% at 4 days, whereas sperm diluted with extenders showed 57.57 +/- 12.33% (E1), 64.34 +/- 11.86% (E2), and 61.40 +/- 12.41% (E3) motility at 6 days. This study explored extenders optimized with higher osmolarity (39-62 mOsm/kg) and lower K+ (1 mmol/L) as the most suitable medium for storing sterlet sperm for 6 days. After 6 days post storage, sperm diluted with extenders E1-E3 achieved a fertilization rate of 31.29 +/- 14.2%, 31.66 +/- 8.84%, and 30.67 +/- 10.02%, respectively, and hatching success of 29.58 +/- 13.4%, 30.50 +/- 7.89%, and 27.95 +/- 9.62%, respectively with freshly ovulated eggs.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10604 - Reproductive biology (medical aspects to be 3)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Fish Physiology and Biochemistry

ISSN

0920-1742

e-ISSN

1573-5168

Volume of the periodical

51

Issue of the periodical within the volume

neuvedeno

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

12

Pages from-to

nestránkováno

UT code for WoS article

001369106400002

EID of the result in the Scopus database

2-s2.0-85211137622