Application of a practical method for short-term stored common carp sperm with extenders and quality adjustment before fertilization for breeding success
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F25%3A43909651" target="_blank" >RIV/60076658:12520/25:43909651 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.anireprosci.2025.107763" target="_blank" >https://doi.org/10.1016/j.anireprosci.2025.107763</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.anireprosci.2025.107763" target="_blank" >10.1016/j.anireprosci.2025.107763</a>
Alternative languages
Result language
angličtina
Original language name
Application of a practical method for short-term stored common carp sperm with extenders and quality adjustment before fertilization for breeding success
Original language description
The main objective of this study was to elucidate whether there are any effects of sperm dilution time before short-term storage in common carp. The sperm of five males (2-2.5 kg body weight, 3 years old) with good spermatozoa motility (>90 %) were diluted with carp extender immediately, 3 h post-diluted, and undiluted fresh sperm was used as control. Sperm motility and velocity of undiluted and diluted sperm were evaluated using a CASA system. A two-way ANOVA revealed that spermatozoa motility, curvilinear velocity (VCL), and straight-line velocity (VSL) were significantly impacted by storage time, varying dilution with extenders, and their interaction (p < 0.001). Sperm motility and velocity dropped steadily with increasing storage periods for diluted sperm, while they drastically fell for undiluted sperm and became immotile at 5 days poststorage. In the control group, the mean percentage of sperm motility was achieved by 40.60 % at 3 days post-storage and sperm diluted with Cejko solution immediately and 3 h post-diluted sperm retained motility at 65.13 % and 65.63 %, respectively, at 3 days post-storage and retained motility of 39.45 and 40.43 %, respectively, at 7 days post-storage. After 7 days poststorage, the diluted (immediately and 3 h post-diluted) pooled sperm had a 61.01 % and 56.70 % fertilization and a 43.40 % and 36.52 % hatching rate, whereas fresh sperm had an 89.34 % fertilization and 78.06 % hatching success. According to the results of the fertilization and hatching assay, compared to fresh sperm, 7 days of stored diluted pooled sperm produced a 68.29 % fertilization rate and a 55.60 % hatching rate.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10604 - Reproductive biology (medical aspects to be 3)
Result continuities
Project
<a href="/en/project/QK21010141" target="_blank" >QK21010141: Management of gametes and reproduction in the protection of genetic resources and the breeding program of common carp in the conditions of aquaculture in the Czech Republic</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Animal Reproduction Science
ISSN
0378-4320
e-ISSN
1873-2232
Volume of the periodical
274
Issue of the periodical within the volume
neuvedeno
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
nestránkováno
UT code for WoS article
001426408100001
EID of the result in the Scopus database
2-s2.0-85217210601