Estimation of rheological parameters for unstained living cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43902667" target="_blank" >RIV/60076658:12520/21:43902667 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1140/epjs/s11734-021-00084-2" target="_blank" >https://doi.org/10.1140/epjs/s11734-021-00084-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1140/epjs/s11734-021-00084-2" target="_blank" >10.1140/epjs/s11734-021-00084-2</a>
Alternative languages
Result language
angličtina
Original language name
Estimation of rheological parameters for unstained living cells
Original language description
In video-records, objects moving in intracellular regions are often hardly detectable and identifiable. To squeeze the information on the intracellular flows, we propose an automatic method of reconstruction of intracellular flow velocity fields based only on a recorded video of an unstained cell. The basis of the method is detection of speeded-up robust features (SURF) and assembling them into trajectories. Two components of motion-direct and Brownian-are separated by an original method based on minimum covariance estimation. The Brownian component gives a spatially resolved diffusion coefficient. The directed component yields a velocity field, and after fitting the vorticity equation, estimation of the spatially distributed effective viscosity. The method was applied to videos of a human osteoblast and a hepatocyte. The obtained parameters are in agreement with the literature data.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10305 - Fluids and plasma physics (including surface physics)
Result continuities
Project
<a href="/en/project/LM2018099" target="_blank" >LM2018099: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Physical Journal Special Topics
ISSN
1951-6355
e-ISSN
1951-6401
Volume of the periodical
230
Issue of the periodical within the volume
4
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
1105-1112
UT code for WoS article
000641242500002
EID of the result in the Scopus database
2-s2.0-85112120239