High-carbohydrate diet altered conversion of metabolites, and deteriorated health in juvenile largemouth bass
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F22%3A43904434" target="_blank" >RIV/60076658:12520/22:43904434 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.aquaculture.2021.737816" target="_blank" >https://doi.org/10.1016/j.aquaculture.2021.737816</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aquaculture.2021.737816" target="_blank" >10.1016/j.aquaculture.2021.737816</a>
Alternative languages
Result language
angličtina
Original language name
High-carbohydrate diet altered conversion of metabolites, and deteriorated health in juvenile largemouth bass
Original language description
To explore the effects of dietary carbohydrate level on nutrients metabolism, largemouth bass juveniles (initial weight, 4.0 & PLUSMN; 0.2 g) were fed three isonitrogenous and isoenergetic diets containing 9.66% (L), 14.32% (M) or 19.11% (H) carbohydrate for 8 weeks. The lowest weight gain (15.75 & PLUSMN; 0.76 g) was observed in group H. Feeding high carbohydrate diet (HCD) led to increased pyruvate (PA), lactic acid (LD), triglyceride (TG) and free fatty acids (NEFA) levels in plasma. Also, HCD enhanced vacuolation, glycogen granule and lipid accumulation in fish liver. Activities of hepatic glycolysis enzymes such as hexokinase, pyruvate kinase and lactate dehydrogenase did not significantly differ among treatments (P > 0.05). HCD resulted in enhancement of hepatic phosphoenolpyruvate carboxykinase (PEPCK) and lipase (LPS) activities, and increased glycogen and triglyceride (TG) concentrations. Similarly, expression of glucose and lipid metabolism related genes such as glycogen phosphorylase (PYG) and carnitine palmitoyl transferases (CPT1, CPT2) were up-regulated with increasing carbohydrate level. Hepatic catalase (CAT) and glutathione peroxidase (GSH-PX) activities, and total antioxidant capacity (T-AOC) were decreased in HCD group. UPLC-MS metabolomics revealed that glucose metabolism, lipid metabolism, and antioxidant defense system were influenced by dietary carbohydrate level. HCD raised the accumulation of carbohydrate metabolites (phosphohydroxypyruvic acid), unsaturated fatty acids (19(R)-HETE, 9(S)-HPOT, alpha-Linolenic acid and oleic acid), cholesterol, and antioxidant functional substance (oxidized glutathione). Additionally, these differential metabolites were enriched in the metabolic pathways such as galactose metabolism, fructose and mannose metabolism, unsaturated fatty acid biosynthesis, primary bile acid biosynthesis, cholesterol-butyrate metabolism, glutathione metabolism, serine and threonine metabolism. Overall, our results revealed the details of metabolites conversion in juvenile largemouth bass fed HCD.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40103 - Fishery
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Aquaculture
ISSN
0044-8486
e-ISSN
1873-5622
Volume of the periodical
549
Issue of the periodical within the volume
neuvedeno
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
nestrankovano
UT code for WoS article
000765399200009
EID of the result in the Scopus database
2-s2.0-85121141127