Bioenergetic pathways during non-activation, post-activation, and reactivation of motility exhausted sterlet (Acipenser ruthenus) spermatozoa

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F23%3A43906209" target="_blank" >RIV/60076658:12520/23:43906209 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.aquaculture.2022.739221" target="_blank" >https://doi.org/10.1016/j.aquaculture.2022.739221</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2022.739221" target="_blank" >10.1016/j.aquaculture.2022.739221</a>

Result language

angličtina

Original language name

Bioenergetic pathways during non-activation, post-activation, and reactivation of motility exhausted sterlet (Acipenser ruthenus) spermatozoa

Original language description

This study studied the possibility of sperm reactivation employing second motility activation, adenosine triphosphate (ATP) reloading, and regaining the fertilizing ability and the possibility of improvement in the fertilisation rate of reactivated sperm by supplementation with metabolites. The most crucial bioenergetic pathways during quiescent, motile, and reactivated states of sterlet (Acipenser ruthenus) spermatozoa were determined. Results showed that out of the studied pathways (glycolysis, mitochondrial respiration, and phosphocreatine-creatine - PCr-Cr shuttle system), PCr-Cr shuttle system was the most requested during the quiescent state. While after motility activation, no studied pathway had a significant energy supply contribution. Thus, the bioenergetic strategy to sustain sperm motility in sturgeon is similar to that in teleostean fishes. After termination of the first round of motility, the spermatozoa of sterlet were able to become motile and fertilise the egg cells after 1-h incubation in a reactivation medium. Mitochondrial respiration and the PCr-Cr shuttle system were shown to be active during the reactivation state. However, unlike spermatozoa of teleostean fishes, the sterlet reactivated spermatozoa did not demonstrate ATP reloading. This may indicate for the first time that the bioenergetic strategy during second motility activation in sturgeon sperm is divergent from cyprinids and sal-monids. The fact that only part of spermatozoa can be reactivated is probably related to the existence of sperm subpopulations of specific intrinsic physiological properties. Further study of these properties will enhance our fundamental knowledge in fish spermatology and help increase the effectiveness of artificial fish reproduction.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10620 - Other biological topics

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Aquaculture

ISSN

0044-8486

e-ISSN

1873-5622

Volume of the periodical

566

Issue of the periodical within the volume

neuvedeno

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

12

Pages from-to

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UT code for WoS article

000936541700001

EID of the result in the Scopus database

2-s2.0-85145740187