Changes in sperm metabolome during carp sperm short-term storage in different media: In search of sperm quality and storage capability markers

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F24%3A43908050" target="_blank" >RIV/60076658:12520/24:43908050 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.aquaculture.2023.740381" target="_blank" >https://doi.org/10.1016/j.aquaculture.2023.740381</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2023.740381" target="_blank" >10.1016/j.aquaculture.2023.740381</a>

Result language

angličtina

Original language name

Changes in sperm metabolome during carp sperm short-term storage in different media: In search of sperm quality and storage capability markers

Original language description

Although short-term hypothermic storage of sperm is already widely used in artificial fish reproduction and is considered very useful for resolving multiple problems related to logistics and broodstock maintenance, this approach continues to be explored and developed. In the present study, targeted and global metabolome LC/MS analyses of common carp sperm during refrigerated storage in undiluted and diluted (in a buffer: NaCl 55 mmol/ L, KCl 40 mmol/L, CaCl2 1 mmol/L, MgSO4 0.5 mmol/L, sucrose 87.5 mmol/L, Tris 10 mmol/L, pH 8, with or without 5% methanol) conditions were used to find potential metabolic markers of fish sperm quality and storage capability evaluated by sperm motility parameters. Sperm motility percentage started to decline from the 6th day of the storage, followed by a significant drop in ATP content in all studied groups, ADP content in undiluted sperm, and creatine phosphate content in sperm diluted by the methanol-containing buffer. Correlation analysis of whole metabolome and sperm motility data allowed us to suggest several potential markers belonging to different chemical classes (mainly nucleotides and their components). Considering motility preservation and changes in the content of proposed markers of sperm quality/storage ability, sperm storage in the methanolcontaining buffer is more promising for further application. At the same time, further searches for reliable markers and their validation remain necessary.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10604 - Reproductive biology (medical aspects to be 3)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Aquaculture

ISSN

0044-8486

e-ISSN

1873-5622

Volume of the periodical

581

Issue of the periodical within the volume

neuvedeno

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

10

Pages from-to

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UT code for WoS article

001122916000001

EID of the result in the Scopus database

2-s2.0-85178264013