Aging oocytes: exploring apoptosis and its impact on embryonic development in common carp (Cyprinus carpio)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F25%3A43909645" target="_blank" >RIV/60076658:12520/25:43909645 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1093/jas/skaf002" target="_blank" >https://doi.org/10.1093/jas/skaf002</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jas/skaf002" target="_blank" >10.1093/jas/skaf002</a>

Result language

angličtina

Original language name

Aging oocytes: exploring apoptosis and its impact on embryonic development in common carp (Cyprinus carpio)

Original language description

Ovulation, fertilization, and embryo development are orchestrated and synchronized processes essential for the optimal health of offspring. Postovulatory aging disrupts this synchronization and impairs oocyte quality. In addition, oocyte aging causes fertilization loss and poor embryo development. This investigation aimed to unravel the endpoint of in vitro oocyte aging in common carp (Cyprinus carpio) to understand the involvement of apoptosis in postovulatory oocyte death. It was observed that the fertilization ability significantly declined (P &lt; 0.001) at 8-h poststripping (HPS), subsequently triggering apoptosis in the advanced stage of oocyte aging, i.e., 48 HPS. This process included an increase in proapoptotic transcripts (fas, bax, cathepsin D, caspase 8, caspase 9, and caspase 3a) (P &lt; 0.05), elevated levels of caspase 3 protein (P &lt; 0.05), and activation of caspase 3 enzyme (P &lt; 0.001), a key player in apoptosis, in aging oocytes. Furthermore, the effects of oocyte aging on the embryonic apoptosis machinery were examined in embryos at 5-h postfertilization (HPF) and 24 HPF derived from fresh and aged oocytes. Expression of apoptotic genes and caspase enzyme activity remained at the basal level in 5 HPF (early blastula embryos) from both fresh and aged oocytes. In contrast, the zymogenic and active forms of caspase 3 increased in 24 HPF embryos from 8-h-aged oocytes (P &lt; 0.01) compared with those from fresh oocytes. Thus, apoptosis intensified in 24 HPF embryos from aged oocytes without affecting the apoptotic machinery of early blastula embryos. Our findings demonstrate that apoptosis initiated by the Fas/FasL system is an important physiological process accompanying oocyte aging in common carp.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10604 - Reproductive biology (medical aspects to be 3)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Animal Science

ISSN

0021-8812

e-ISSN

1525-3163

Volume of the periodical

103

Issue of the periodical within the volume

neuvedeno

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

nestránkováno

UT code for WoS article

001405329800001

EID of the result in the Scopus database

2-s2.0-85216479318