The Src family kinases contribute to MII arrest maintenance in aging porcine oocytes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F25%3A102382" target="_blank" >RIV/60460709:41210/25:102382 - isvavai.cz</a>
Alternative codes found
RIV/00027014:_____/25:10006344
Result on the web
<a href="https://doi.org/10.1016/j.theriogenology.2025.117614" target="_blank" >https://doi.org/10.1016/j.theriogenology.2025.117614</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.theriogenology.2025.117614" target="_blank" >10.1016/j.theriogenology.2025.117614</a>
Alternative languages
Result language
angličtina
Original language name
The Src family kinases contribute to MII arrest maintenance in aging porcine oocytes
Original language description
Mature mammalian oocytes arrest meiosis in metaphase II (MII). If the oocyte is not fertilized, it can spontaneously break the MII arrest. Spontaneous activation and postovulatory aging hinder precisely timed and regulated embryonic development. To elucidate the role of Src family protein tyrosine kinases (SFKs) in porcine oocyte MII arrest, activation, and aging, we used a specific SFK inhibitor and immunolocalization. The 24h-prolonged oocyte culture in the presence of SFK inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d] pyrimidine (PP2) increased (P < 0.05) the proportion of spontaneously activated porcine oocytes compared to controls. Further culture with PP2 inhibitor led to an increase (P < 0.05) in the parthenogenetic embryos and a decrease (P <0.05) in lytic oocytes. SFK inhibition did not affect (P > 0.05) the proportion of ionophore A23187activated oocytes. SFKs were localized in the perichromosomal region, in the pronuclei, in the cytoplasm, and on the plasma membrane of oocytes and parthenogenetic embryos after 24, 48, and 72 h of prolonged in vitro culture. The greatest SFKs fluorescence was detected after a 24h-prolonged culture on the plasma membrane of MII oocytes. In embryos and fragmented oocytes, intense fluorescence was detected in the cleavage furrow region and on the membrane of apoptotic vesicles, respectively. Our results reveal the involvement of SFKs in MII arrest maintenance, though they don't appear to modulate the early processes of ionophore-stimulated parthenogenetic activation. Changes in the distribution of SFKs during prolonged culture suggest their role in signaling cascades associated with actin filament cytoskeleton organization.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Theriogenology
ISSN
0093-691X
e-ISSN
1879-3231
Volume of the periodical
248
Issue of the periodical within the volume
neuvedeno
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
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UT code for WoS article
001545893600001
EID of the result in the Scopus database
2-s2.0-105012131274