Src family kinases are involved in the meiotic maturation of porcine oocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F15%3A%230002259" target="_blank" >RIV/00027014:_____/15:#0002259 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41210/15:68120

Result on the web

<a href="http://www.vuzv.cz/sites/File/_privat/15110.pdf" target="_blank" >http://www.vuzv.cz/sites/File/_privat/15110.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Src family kinases are involved in the meiotic maturation of porcine oocytes

Original language description

Mammalian meiotic maturation is regulated by changes in the phosphorylation state of proteins involved in signalling pathways. The regulatory proteins include the family of Src tyrosine kinases. Src family kinases (SFKs) are required for meiotic maturation of mouse oocytes, and it remains to be elucidated whether they play the same role in porcine oocytes. To clarify the role of SFKs in the meiotic maturation of porcine oocytes we used inhibition of SFKs, western blotting and immunolocalisation to determine the presence of SFKs and localisation in the oocytes and assays to determine the activity of maturation-promoting factor (MPF) and mitogen-activated protein kinase (MAPK). Inhibition of SFKs resulted in the disruption of oocyte maturation and led to a decline in MPF and MAPK activity. The fluorescence intensity of SFKs in the cytoplasm and membrane of MI oocytes decreased significantly compared with germinal vesicle oocytes. The highest fluorescence intensity for SFKs was detected on the membrane of MII oocytes. Only weak fluorescence was detected in the perichromosomal area of MI and MII oocytes. These results prove that SFKs play an active role in the meiotic maturation of porcine oocytes by regulating MPF and MAPK activity.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GG - Zootechnics

OECD FORD branch

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Project

<a href="/en/project/QI101A166" target="_blank" >QI101A166: Biotechnology in pig husbandry and breeding</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Reproduction Fertility and Development

ISSN

1031-3613

e-ISSN

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Volume of the periodical

27

Issue of the periodical within the volume

7

Country of publishing house

AU - AUSTRALIA

Number of pages

9

Pages from-to

1097-1105

UT code for WoS article

000360395200013

EID of the result in the Scopus database

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