Effect of reduced osmolality in glucose-based extenders on the post-thaw motility of Mediterranean brown trout (Salmo cettii) spermatozoa
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F25%3A43909841" target="_blank" >RIV/60076658:12520/25:43909841 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.3389/fmars.2025.1621496" target="_blank" >https://doi.org/10.3389/fmars.2025.1621496</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fmars.2025.1621496" target="_blank" >10.3389/fmars.2025.1621496</a>
Alternative languages
Result language
angličtina
Original language name
Effect of reduced osmolality in glucose-based extenders on the post-thaw motility of Mediterranean brown trout (Salmo cettii) spermatozoa
Original language description
The current study aimed to compare the effects of decreasing osmolality in glucose-based extenders containing methanol alone versus a methanol-ethylene glycol combination on the post-thaw motility of Mediterranean brown trout (Salmo cettii) spermatozoa. Milt was collected from mature males in the Biferno River, Southern Italy, and cryopreserved using a control cryomedium (150 mM glucose with 7.5% methanol) and experimental variants with reduced glucose (100 mM, 50 mM) paired with methanol alone or combined with 2.5% ethylene glycol. Samples were cryopreserved in 0.25 mL straws with a final sperm concentration of 3.0 x 10(9) spermatozoa/mL, corresponding to 750 x 10(6) spermatozoa/straw. Sperm motility and kinematic parameters of Mediterranean brown trout were assessed for fresh sperm and post-thaw samples cryopreserved in various cryomedia. Fresh sperm exhibited robust motility (89.0 +/- 8.4%) and curvilinear velocity (VCL, 121.2 +/- 22.4 mu m/s), while the control cryomedium preserved motility at 65.9 +/- 12.9% and VCL at 117.6 +/- 26.1 mu m/s; the experimental 100 mM glucose with 7.5% methanol and 2.5% ethylene glycol treatment yielded comparable motility (65.6 +/- 11.6%) but reduced VCL (81.5 +/- 16.1 mu m/s, p < 0.05). Other treatments showed diminished efficacy, particularly at 50 mM glucose. These findings reveal that a hypotonic extender with methanol-ethylene glycol synergy sustains motility after cryopreservation despite lower glucose levels, demonstrating that the osmotic threshold of extender can be lowered to 100 mOsm/kg without compromising salmonid sperm function. This approach offers a practical tool for conserving S. cettii, supporting broader restoration efforts amid escalating environmental pressures.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40103 - Fishery
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Marine Science
ISSN
2296-7745
e-ISSN
2296-7745
Volume of the periodical
12
Issue of the periodical within the volume
neuvedeno
Country of publishing house
CH - SWITZERLAND
Number of pages
7
Pages from-to
nestránkováno
UT code for WoS article
001549653900001
EID of the result in the Scopus database
2-s2.0-105012953963