Exploring cryomedium with hypotonic extender for sturgeon sperm cryopreservation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F25%3A43909712" target="_blank" >RIV/60076658:12520/25:43909712 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.aquaculture.2025.742497" target="_blank" >https://doi.org/10.1016/j.aquaculture.2025.742497</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2025.742497" target="_blank" >10.1016/j.aquaculture.2025.742497</a>

Result language

angličtina

Original language name

Exploring cryomedium with hypotonic extender for sturgeon sperm cryopreservation

Original language description

This study investigates sperm cryopreservation across multiple sturgeon species (sterlet, Siberian, Russian, and stellate sturgeons), testing different cryomedia and two cryopreservation formats: small 0.5 ml plastic straws, rapidly cooled on a polystyrene raft within a Styrofoam box, and larger 4.5 ml and 10 ml cryotubes subjected to a controlled, slow cooling process in a programmable freezer. The cryomedia tested included a novel hypotonic (relative to the osmolality of seminal plasma) extender containing 1 mM KCl, methanol, and ethylene glycol at varying concentrations, alongside traditional cryomedium. The study assessed the impact of cryomedia on spermatozoa volume changes during the equilibration period, fertilization success, and embryogenesis. Notably, the cryoprotectants ethylene glycol and methanol affected sperm cell volume differently: ethylene glycol caused initial shrinkage, followed by volume normalization, whereas methanol did not induce significant changes in spermatozoa volume. Key findings demonstrated that a cryomedium with a hypotonic extender containing 1 mM KCl, 11 % methanol, and 2.5 % ethylene glycol effectively preserved post-thaw sperm motility, achieving 46 f 6 % in both 0.5 ml straws and 4.5 ml cryotubes for Siberian sturgeon; 31 f 10 % in 0.5 ml straws and 40 f 12 % in 10 ml cryotubes for Russian sturgeon; and 46 f 10 % in 0.5 ml straws, 39 f 8 % in 4.5 ml cryotubes, and 44 f 11 % in 10 ml cryotubes for Stellate sturgeon. The cryomedium also supported high embryo development and hatching rates, with Siberian sturgeon achieving 81 f 16 % and 79 f 15 % in 0.5 ml straws, and 84 f 6 % and 82 f 7 % in 4.5 ml cryotubes. For Russian sturgeon, development and hatching rates were 60 f 20 % and 57 f 20 % in 0.5 ml straws, 51 f 12 % and 48 f 13 % in 4.5 ml cryotubes, and 62 f 11 % and 48 f 13 % in 10 ml cryotubes. These results highlight the effectiveness of this alternative cryopreservation approach across different sturgeon species and sample volumes. However, spermatozoa from cryopreserved samples with the 1 mM KCl extender showed a statistically significant reduction in curvilinear velocity (VCL) compared to fresh sperm and spermatozoa cryopreserved in traditional cryomedia. Nevertheless, fertilization success was not affected, and no significant morphological abnormalities were observed in the resulting embryos. This study introduces an alternative cryopreservation protocol for sturgeon species, demonstrating the feasibility of using cryomedium with 1 mM KCl extender and large sample volumes (4.5 ml and 10 ml cryotubes) without compromising fertilization outcomes. The findings contribute to advancing cryopreservation techniques, offering promising new possibilities for sturgeon aquaculture and conservation.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40103 - Fishery

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Aquaculture

ISSN

0044-8486

e-ISSN

1873-5622

Volume of the periodical

605

Issue of the periodical within the volume

neuvedeno

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

13

Pages from-to

nestránkováno

UT code for WoS article

001465051500001

EID of the result in the Scopus database

2-s2.0-105001712038