Cryopreservation of common carp ( Cyprinus carpio L.) sperm: Insights into low-ionic cryoprotective medium mechanism and efficacy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F25%3A43909711" target="_blank" >RIV/60076658:12520/25:43909711 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.aquaculture.2024.741807" target="_blank" >https://doi.org/10.1016/j.aquaculture.2024.741807</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2024.741807" target="_blank" >10.1016/j.aquaculture.2024.741807</a>

Result language

angličtina

Original language name

Cryopreservation of common carp ( Cyprinus carpio L.) sperm: Insights into low-ionic cryoprotective medium mechanism and efficacy

Original language description

The report explaining the processes and conditions common carp spermatozoa required for successful cryopreservation using a low-ionic cryoprotective medium was represented. The study aimed at the cryopreservation outcomes of common carp sperm using hypertonic and hypotonic cryoprotective media, focusing on spermatozoon volume changes during the equilibration period and offspring ploidy levels. The effect of two types of cryoprotective media high-ionic (200 mM glucose, 40 mM KCl, 30 mM TRIS, 10 % methanol) and low-ionic (60 mM NaCl, 2.9 mM sucrose, 11 % methanol, 2.5 % ethylene glycol), two volumes of samples and two different cryopreservation methods (0.5 ml straws on a polystyrene raft in a Styrofoam box with rapid cooling rates and 4.5 ml cryotubes in the programmable freezer with slow cooling rates) were assessed. The best post-thawed motility results of common carp spermatozoa after hypertonic shrinking in straws with high-ionic cryoprotective medium, a dilution ratio of 1:9 (44.91 +/- 9.98 %), and hypotonic swelling in cryotubes with low-ionic cryoprotective medium, a dilution ratio of 1:1 (47,33 +/- 7.85 %) were obtained. The key to successful sperm cryopreservation is matching a cryoprotective medium and cooling rates to maintain osmotic balance and protect cells against mechanical damage from ice crystals. The slow cooling rate and the synergy effect of methanol and ethylene glycol make it possible to apply a low-ionic cryoprotective medium in a big sample volume for cryopreservation of common carp sperm. No significant differences in malformations or ploidy levels were observed between larvae from fresh and cryopreserved sperm. These findings underscore the potential and benefits of applying a low-ionic cryopreservation medium for common carp artificial reproduction in aquaculture.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40103 - Fishery

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Aquaculture

ISSN

0044-8486

e-ISSN

1873-5622

Volume of the periodical

596

Issue of the periodical within the volume

neuvedeno

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

nestránkováno

UT code for WoS article

001350198000001

EID of the result in the Scopus database

2-s2.0-85207776428