Quantitative analysis of neurotransmitters in fish brain: A tool to assess neurochemical effects of psychoactive pollutants
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F25%3A43910037" target="_blank" >RIV/60076658:12520/25:43910037 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.envpol.2025.127031" target="_blank" >https://doi.org/10.1016/j.envpol.2025.127031</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.envpol.2025.127031" target="_blank" >10.1016/j.envpol.2025.127031</a>
Alternative languages
Result language
angličtina
Original language name
Quantitative analysis of neurotransmitters in fish brain: A tool to assess neurochemical effects of psychoactive pollutants
Original language description
Psychoactive drugs in fish act through mechanisms similar to those in humans because their main neurotransmitter (NT) systems are analogous. Psychoactive drugs are ubiquitous in aquatic environments, having negative effects on fish and other aquatic organisms. To understand the neurochemical changes underlying the effects, studying neurotransmitter levels is essential. The detection of NTs and their metabolites in biological samples may be challenging. A new analytical method using hydrophilic interaction liquid chromatography coupled to high-resolution mass spectrometry was developed. The method includes simple extraction and 17 min analytical run and enables reliable quantification of a wide range of NTs from different classes, including their precursors and metabolites: acetylcholine, choline, citrulline, 3,4-dihydroxyphenylacetic acid, dopamine, dopamine-3-O-sulfate, epinephrine, gamma-aminobutyric acid, gamma-hydroxybutyric acid, glutamate, glutamine, alpha-glycerophosphorylcholine, guanidine, histamine, histidine, levodopa, melatonin, norepinephrine, normetanephrine, octopamine, phenylalanine, phosphocholine, serotonin, 5-hydroxyindoleacetic acid, synephrine, tryptophan, 5-hydroxytryptophan, tyramine, 3-methoxytyramine, and tyrosine. The method was validated with highly satisfying performance parameters regarding sensitivity, repeatability and trueness. Under optimized conditions, recoveries from 55 to 130 % were obtained, while intra-day and inter-day RSD never exceeded 24 and 29 %, respectively. LOQs between 0.0012 and 25 mu g/g d.w. were achieved. Finally, the optimized method was applied to analyze NTs in the brains of two fish species: European perch and rainbow trout. Proposed method will help to obtain fundamental information on NTs content in fish brain and on their changes in response to the exposure to psychoactive drugs.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA22-03754S" target="_blank" >GA22-03754S: From brain to sperm: how psychoactive pollutants can affect fish sperm function.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Environmental Pollution
ISSN
0269-7491
e-ISSN
1873-6424
Volume of the periodical
384
Issue of the periodical within the volume
neuvedeno
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
nestránkováno
UT code for WoS article
001561943500001
EID of the result in the Scopus database
2-s2.0-105013851869