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Monoclonal antibody FsC-47 against carp sperm creatine kinase

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12610%2F06%3A00006945" target="_blank" >RIV/60076658:12610/06:00006945 - isvavai.cz</a>

  • Alternative codes found

    RIV/61388971:_____/06:00048372 RIV/68378050:_____/06:00048372

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Monoclonal antibody FsC-47 against carp sperm creatine kinase

  • Original language description

    The enzyme creatine kinase (CK) plays a key role in energy homeostasis of cells with high and fluctuating energy requirements. As for spermatozoa, the activity of phosphocreatine shuttle, which directs energy from the mitochondria to sites of ATP consumption, is dependent on individual species. High activities of CK are observed in spermatozoa of nonvertebrate, fish, and birds, contrary to the low-level CK activity in mammalian spermatozoa. A new monoclonal antibody (MAb) to carp sperm creatine kinase was prepared. This antipody is applicable to large-scale immunochemical techniques. In this study, it was applied to the study of carp sperm motility, and the evaluation of the influence of CK on the quality and fertilization ability of carp spermatozoa.

  • Czech name

    Monoklonalní protilátky FsC-47 proti kapří kreatine kinase

  • Czech description

    Enzym kreatine kinasa (CK) hraje důležitou roli v energetické stabilitě buněk vyznačující se požadovaly na vysokou dostupnost CK. V případě spermií je aktivita energetického transportu phosphocreatinem směřující z mitochondrie do místa spotřeby ATP je vždy závislá na druhové příslušnosti. Vysoká aktivita CK byla nalezena u spermií bezobratlých, ryb a ptáků ve srovnání s nízkou úrovní aktivity CK aktivity u savčích spermií. Nové monoklonální protilátky (MAb) byly připraveny u kreatine kinasy kapřího spermatu a aplikovány širokou immunochemickou technikou. Ve studii byly sledována úroveň CK kapřích spermií ve vztahu k jejich úrovni motility a oplozovací schopnosti.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    GL - Fishery

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2006

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Hybridoma

  • ISSN

    1554-0014

  • e-ISSN

  • Volume of the periodical

    25

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    4

  • Pages from-to

    154-157

  • UT code for WoS article

  • EID of the result in the Scopus database