Quality assessment of cryopreserved black-lip pearl oyster Pinctada margaritifera spermatozoa
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F18%3A43898754" target="_blank" >RIV/60076658:12520/18:43898754 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0044848617325620" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0044848617325620</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aquaculture.2018.07.067" target="_blank" >10.1016/j.aquaculture.2018.07.067</a>
Alternative languages
Result language
angličtina
Original language name
Quality assessment of cryopreserved black-lip pearl oyster Pinctada margaritifera spermatozoa
Original language description
High quality of sperm is essential to a high fertilization rate, especially post- cryopreservation. Assessment of sperm integrity, motility and energy reserves before cryopreservation is necessary for selection of milt with optimal fertilizing potential. We describe the effect of cryopreservation on the quality of black-lip pearl oyster, Pinctada margaritifera var. cumingii sperm. Evaluated quality indices of fresh and frozen/thawed P. margaritifera spermatozoa, included morphology, ultrastructure and motility characteristics relative to the energy content (ATP) and its capacity to be sustained by mitochondrial respiration. Morphology and ultrastructure were quantitatively evaluated using images obtained by optical microscopy assisted by the Image J software and TEM, respectively. Sperm motility was assessed using Image J software combined with a computer assisted sperm analysis plugin adapted for assessing P. margaritifera spermatozoa. Other sperm quality parameters evaluated included O-2 consumption, ATP content, and creatine kinase activity. Frozen/thawed spermatozoa exhibited damage to the head but retained a compact spherical shape. Sperm motility indicators showed a significant decrease in quality resulting from the freeze/thaw process. The percent of motile cells was 54% compared to 84% in fresh sperm, O-2 consumption was 4.8 compared to 44 nanomol min(-1), ATP content was 0.72 nmol/10(9) spermatozoa in the activating medium compared to 4.54 nmol/10(9) spermatozoa, and creatine kinase activity was 9.06 x 10(-5) IU mg(-1) protein compared to 12.5 x 10(-5) IU mg(-1) protein. The cryopreservation protocol allowed obtaining an acceptable motility rate after thawing, confirming the predictive value of sperm motility measurements before cryopreservation in terms of their ability to withstand freezing process.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10604 - Reproductive biology (medical aspects to be 3)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Aquaculture
ISSN
0044-8486
e-ISSN
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Volume of the periodical
497
Issue of the periodical within the volume
DEC 2018
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
278-286
UT code for WoS article
000442900300035
EID of the result in the Scopus database
2-s2.0-85051024839