Sequential assembly of photosynthetic units in Rhodobacter sphaeroides as revealed by fast repetition rate analysis of variable bacteriochlorophyll a fluorescence

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12640%2F05%3A00006488" target="_blank" >RIV/60076658:12640/05:00006488 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Sequential assembly of photosynthetic units in Rhodobacter sphaeroides as revealed by fast repetition rate analysis of variable bacteriochlorophyll a fluorescence

Original language description

The development of functional photosynthetic units in Rhodobacter sphaeroides was followed by near infra-red fast repetition rate (IRFRR) fluorescence measurements that were correlated to absorption spectroscopy, electron microscopy and pigment analyses.To induce the formation of intracytoplasmic membranes (ICM) (greening), cells grown aerobically both in batch culture and in a carbon-limited chemostat were transferred to semiaerobic conditions. In both aerobic cultures, a low level of photosynthetic complexes was observed, which were composed of the reaction center and the LH1 core antenna. Interestingly, in the batch cultures the reaction centers were essentially inactive in forward electron transfer and exhibited low photochemical yields F-v/F-M, whereas the chemostat culture displayed functional reaction centers with a rather rapid (1-2 ms) electron transfer turnover, as well as a high F-v/F-M of similar to0.8. In both cases, the transfer to semiaerobiosis resulted in rapid induct

Czech name

Postupné sestavování fotosyntetických jednotek u Rhodobacter sphaeroides studované pomocí Fast repetition rate analýzy variabilní fluorescence bakteriochlororofylu a.

Czech description

Sestavování fotosyntetických jednotek u Rhodobacter sphaeroides bylo studováno pomocí analýzy kinetických změn variabilní fluorescence bakteriochlororofylu a. Sestavování fotosyntetických komplexů bylo indukováno přenesením aerobní kultury Rh. sphaeroides do semiaerobních podmínek. Analýza na povídá, že nejdříve jsou sestavována reakční centra, poté vnitřní anténa LH1, poté je aktivován elektronový transport a na závěr se sestavuje vnější světlosběrná anténa LH2.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

<a href="/en/project/LN00A141" target="_blank" >LN00A141: Mechanism, ecophysiology and biotechnology of photosynthesis</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochimica et Biophysica Acta - Bioenergetics

ISSN

0005-2728

e-ISSN

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Volume of the periodical

1706

Issue of the periodical within the volume

3

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

12

Pages from-to

220-231

UT code for WoS article

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EID of the result in the Scopus database

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