Early detection of bean infection by Pseudomonas syringae in asymptomatic leaf areas using chlorophyll fluorescence imaging

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12640%2F08%3A00009365" target="_blank" >RIV/60076658:12640/08:00009365 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Early detection of bean infection by Pseudomonas syringae in asymptomatic leaf areas using chlorophyll fluorescence imaging

Original language description

Chlorophyll fluorescence imaging has been used to analyse the response elicited in Phaseolus vulgaris after inoculation with Pseudomonas syringae pv. phaseolicola 1448A (compatible interaction) and P. syringae pv. tomato DC3000 (incompatible interaction). With the aim of modulating timing of symptom development, different cell densities were used to inoculate bean plants and the population dynamics of both bacterial strains was followed within the leaf tissue. Fluorescence quenching analysis was carriedout and images of the different chlorophyll fluorescence parameters were obtained for infected as well as control plants at different timepoints post-infection. Among the different parameters analysed, we observed that non-photochemical quenching maximised the differences between the compatible and the incompatible interaction before the appearance of visual symptom. A decrease in non-photochemical quenching, evident in both infiltrated and non-infiltrated leaf areas, was observed in P.

Czech name

Včasná detekce infekce u fazolu způsobená Pseudomonas syringae v asymptomatických oblastech listu za pomoci zobrazovací chlorofylové fluorimetrie.

Czech description

Zobrazovací chlorofylová fluorimetrie byla použita k rozlišení kompatibilní a nekompatibilní interakce rostlina ? bakterie v asymptomatickém pletivu fazolu Phaseolus vulgaris infikovaném bakteriemi Pseudomonas syringae pv. phaseolicola 1448A (kompatibilní interakce) and Pseudomonas syringae pv. tomato DC3000 (nekompatibilní interakce). Fazole jsme infikovali dvěma různými koncentracemi buněk bakterií a sledovali jsme vývoj symptomů infekce a populační dymaniku růstu obou kmenů bakterie v rostlinném pletivu. Největší rozdíl ve fluorescenční emisi infikovaného pletiva oběma kmeny bakterie byl v nefotochemickém zhášení fluorescence.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Photosynthesis Research

ISSN

0166-8595

e-ISSN

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Volume of the periodical

96

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

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UT code for WoS article

000253529500005

EID of the result in the Scopus database

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