PRINS on Plant Chromosomes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F06%3A00356458" target="_blank" >RIV/60077344:_____/06:00356458 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/06:00356458

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

PRINS on Plant Chromosomes

Original language description

The introduction of primed in situ labeling (PRINS) into plant cytogenetics provided a novel means for fast and highly specific visualization of DNA sequences on chromosomes and in interphase nuclei. Although the technique does not reach the sensitivityof fluorescence in situ hybridization that is needed for detection of single-copy targets, it is superior in its speed and simplicity. Thus, the main applications of PRINS include fluorescent labeling of repeated DNA sequences, such as ribosomal DNA andsatellite repeats, which are used to discriminate individual chromosome types within karyotypes and to assess the purity of chromosome fractions separated by flow-sorting. Recently, an application of PRINS for the discrimination of sequence subfamilies of satellite repeats has been developed that takes advantage of the sensitivity of Taq polymerase to mismatches between 3?-end of the primer and the template sequences.

Czech name

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Czech description

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Type

C - Chapter in a specialist book

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name

PRINS and In Situ PCR Protocols

ISBN

978-1-58829-549-1

Number of pages of the result

7

Pages from-to

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Number of pages of the book

264

Publisher name

Humana Press

Place of publication

Totowa, NJ

UT code for WoS chapter

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