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Gas chromatographic-mass spectrometric analysis of biomarkers related to folate and cobalamin status in human serum after dimercaptopropanesulfonate reduction and heptafluorobutyl chloroformate derivatization

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F08%3A00310218" target="_blank" >RIV/60077344:_____/08:00310218 - isvavai.cz</a>

  • Alternative codes found

    RIV/00843989:_____/08:00015978

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Gas chromatographic-mass spectrometric analysis of biomarkers related to folate and cobalamin status in human serum after dimercaptopropanesulfonate reduction and heptafluorobutyl chloroformate derivatization

  • Original language description

    Methylmalonic acid and total homocysteine belong to useful clinical indicators of cobalamin and folate status and are commonly measured separately. A sensitive and rapid method has been developed for simultaneous determination of both biomarkers and related metabolites in serum or plasma by isotope dilution gas chromatography?mass spectrometry. Thiols bound in disulfide bonds were released with 2, 3-dimercapto-1-propanesulfonic acid (DMPS), and after deproteinization, they were phase-transfer derivatized with heptafluorobutyl chloroformate in a single step. The reducing capability of the DMPS agent was comparable to that of the dithiothreitol, but exceeded the latter in much cleaner extracts obtained. The new method enabled GC-MS screening of aminoacidic metabolites as their N(S,O)-heptafluorobutoxycarbonyl heptafluorobutyl ester derivatives in serum or plasma. Accurate quantitation of seven biomarkers was accomplished by using deuterated internal standards.

  • Czech name

    Analýza biomarkrů folátového a kobalaminového stavu v lidském séru po redukci dimerkaptopropansulfonátem a derivatizaci heptafluorobutylchlormravenčanem plynovou chromatografií s hmotnostní detekcí

  • Czech description

    Kyselina malonová a celkový homocystein patří k důležitým indikátorům stavu kobalaminu a folátů v organismu a dosud byly měřeny odlišnými metodami. Byla vyvinuta citlivá a rychlá metoda stanovení obou biomarkrů a s nimi souvisejících metabolitů v séru aplasmě plynovou chromatograffí s hmotnostní detekcí. Thiolové vazby v disulfidech byly uvolněny kyselinou 2, 3-dimerkapto-1-propansulfonovou (DMPS) a po deproteinaci byly derivatizovány heptafluorbutyl chlormravenčanem jednostupňovou reakcí. Redukční schopnosti DMPS byly srovnatelné s dithiothreitolem, ovšem DMPS poskytovalo mnohem čistější produkty reakce. Nová metoda umožnila GC-MS kvantifikaci sedmi metabolitů aminokyselin jako jejich N(S,O)-heptafluorobutoxykarbonylheptafluorobutyl esterů v séru a plasmě s použitím deuterovaných vnitřních standardů.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA303%2F06%2F1674" target="_blank" >GA303/06/1674: MULTICOMPLEX DIAGNOSTICS OF B-VITAMIN BIOMARKERS AND NUTRITIONAL STATUS IN PLASMA MICROSAMPLES USING A SIMPLE SAMPLE PRETREATMENT</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Analytical Chemistry

  • ISSN

    0003-2700

  • e-ISSN

  • Volume of the periodical

    80

  • Issue of the periodical within the volume

    15

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    7

  • Pages from-to

  • UT code for WoS article

    000258096700020

  • EID of the result in the Scopus database