GC separation of amino acid enantiomers via derivatization with heptafluorobutyl chloroformate and Chirasil-L-Val column

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F09%3A00335962" target="_blank" >RIV/60077344:_____/09:00335962 - isvavai.cz</a>

Alternative codes found

RIV/00843989:_____/09:00100817

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

GC separation of amino acid enantiomers via derivatization with heptafluorobutyl chloroformate and Chirasil-L-Val column

Original language description

Heptafluorobutyl chloroformate, a recently introduced derivatization reagent, was examined in enantioseparation of amino acids by gas chromatography. Twenty proteinogenic amino acids, plus ornithine, cystine and 4-fluorophenylalanine (internal standard)were treated with the reagent and separation properties of the derivatives assessed on a Chirasil-Val capillary column. Nineteen amino acid enantiomers were efficiently separated in 43 minutes except proline, arginine and cystine. The heptafluorobutyl chloroformate derivatives of the studied DL-amino acids show improved separation over other chloroformate-based derivatives hitherto reported. A combination of the improved and faster separation with a simple derivatization protocol, involving an immediateone-step reaction-extraction in two-phase aqueous-organic medium, and low elution temperatures extend application of heptafluorobutyl chloroformate to chiral amino acid analysis.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA203%2F09%2F2014" target="_blank" >GA203/09/2014: New approaches to alkylchloroformate-mediated sample preparation for parallel GC-MS and LC-MS for metabolite analysis in biofluids</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Volume of the periodical

32

Issue of the periodical within the volume

22

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

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UT code for WoS article

000273993500008

EID of the result in the Scopus database

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