Heptafluorobutyl chloroformate - based sample preparation protocol for chiral and nonchiral amino acid analysis by gas chromatography

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00369909" target="_blank" >RIV/60077344:_____/12:00369909 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-61779-445-2_13" target="_blank" >http://dx.doi.org/10.1007/978-1-61779-445-2_13</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-61779-445-2_13" target="_blank" >10.1007/978-1-61779-445-2_13</a>

Result language
angličtina
Original language name
Heptafluorobutyl chloroformate - based sample preparation protocol for chiral and nonchiral amino acid analysis by gas chromatography
Original language description
Gas chromatography (GC) is a commonly used technique in amino acid analysis (AAA). However, one of the requirements of the application of GC for AAA is a need for the polar analytes to be converted into their volatile, thermally stable derivatives. In the last two decades, alkyl chloroformates have become attractive derivatization reagents. The reagents react immediately with most amino acid functional groups in aqueous matrices and the process can easily be coupled with liquid?liquid extraction of theresulting less-polar derivatives into immiscible organic phase. Here, we describe a simple protocol for in situ derivatization of amino acids with heptafluorobutyl chloroformate followed by subsequent chiral as well as nonchiral GC/mass spectrometric analysis on a respective nonpolar fused silica and an enantioselective Chirasil-Val capillary column.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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