Functional expression and characterization of cathepsin B and L from the gut of the tick Ixodes ricinus

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F09%3A00336472" target="_blank" >RIV/60077344:_____/09:00336472 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/09:00336472
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Functional expression and characterization of cathepsin B and L from the gut of the tick Ixodes ricinus
Original language description
Ticks differ from hematophagous insects in intracellular localization of hemoglobin proteolysis. We have recently described in the hard tick Ixodes ricinus that this process occurs at acidic pH and as is maintained by a machinery of gut-associated cysteine and aspartic proteases similar to blood feeding platyhelmints and nematodes. In this work, we functionally expressed in Pichia pastoris and characterized two components of tick hemoglobinolytic cascade, namely IrCB and IrCL (cysteine proteases of CA family). Using a positional scanning of synthetic combinatorial library we identified the P1?P4 specifities of both enzymes. IrCL displays a strict acidic optimum at pH 4, while IrCB shows broader pH range. Both fusion enzymes were demonstrated to digestbovine hemoglobin and albumin in vitro. Expression profiles of both enzymes were strongly upregulated during blood meal uptake.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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