Breast cancer-specific mutations in CK1epsilon inhibit Wnt/beta-catenin and activate the Wnt/Rac1/JNK and NFAT pathways to decrease cell adhesion and promote cell migration

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F10%3A00352336" target="_blank" >RIV/60077344:_____/10:00352336 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/10:00352336 RIV/60076658:12310/10:00012040 RIV/00216224:14310/10:00067265

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Breast cancer-specific mutations in CK1epsilon inhibit Wnt/beta-catenin and activate the Wnt/Rac1/JNK and NFAT pathways to decrease cell adhesion and promote cell migration

Original language description

In silico modeling and in vivo data showed that autophosphorylation at Thr 44, a site adjacent to the breast cancer point mutations in the N-terminal lobe of human CK1-epsilon, is involved in positive regulation of the CK1-epsilon activity. Our data further demonstrate that, in mammalian cells, mutated forms of CK1-epsilon failed to affect the intracellular localization and phosphorylation of Dvl2; we were able to demonstrate that CK1? mutants were unable to enhance Dvl-induced TCF/LEF-mediated transcription, that CK1-epsilon mutants acted as loss-of-function in the Wnt/beta-catenin pathway, and that CK1-epsilon mutants activated the noncanonical Wnt/Rac-1 and NFAT pathways, similar to pharmacological inhibitors of CK1. In line with these findings, inhibition of CK1 promoted cell migration as well as decreased cell adhesion and E-cadherin expression in the breast cancer-derived cell line MCF7.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Breast Cancer Research

ISSN

1465-5411

e-ISSN

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Volume of the periodical

2010

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

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UT code for WoS article

000285689000006

EID of the result in the Scopus database

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