Toward the crystallization of photosystem II core complex from Pisum sativum L

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F10%3A00355837" target="_blank" >RIV/60077344:_____/10:00355837 - isvavai.cz</a>

Alternative codes found

RIV/67179843:_____/10:00355837 RIV/68378050:_____/10:00355837 RIV/60076658:12640/10:00012204

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Toward the crystallization of photosystem II core complex from Pisum sativum L

Original language description

The crystallization of the higher plant similar to 1 gigadalton homodimeric lipid-pigment-protein complex of photosystem II constitutes a challenging undertaking that has not been crowned with success to date. In the continuation of our previous efforts(Kuta-Smatanova et al. Photosyn. Res. 2006, 90, 255-259) that had provided needle and plate crystals of plant photosystem II for the first time, we report on a study to crystallize three-dimensional crystals of the photosystem 11 from Pisum sativum L. suitable for X-ray analysis. In our screening, we optimized temperature, supersaturation rate, and concentrations of detergents, buffers, salts, and organic additives. We used a sitting and hanging drop vapor-diffusion method, microbatch under oil and counter-diffusion techniques, and direct and cross-influenced procedures. We have identified that the major limiting factors for the growth of crystals are the photosystem II stability and requirement of indispensable divalent cations.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/LC06010" target="_blank" >LC06010: Center of Biocatalysis and Biotransformation</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Crystal Growth & Design

ISSN

1528-7483

e-ISSN

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Volume of the periodical

10

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

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UT code for WoS article

000280471700017

EID of the result in the Scopus database

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