Antiviral activity of tenofovir against Cauliflower mosaic virus and its metabolism in Brassica pekinensis plants
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00367346" target="_blank" >RIV/60077344:_____/11:00367346 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/11:00367346
Result on the web
<a href="http://dx.doi.org/10.1016/j.antiviral.2011.08.014" target="_blank" >http://dx.doi.org/10.1016/j.antiviral.2011.08.014</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.antiviral.2011.08.014" target="_blank" >10.1016/j.antiviral.2011.08.014</a>
Alternative languages
Result language
angličtina
Original language name
Antiviral activity of tenofovir against Cauliflower mosaic virus and its metabolism in Brassica pekinensis plants
Original language description
The antiviral effect of the acyclic nucleoside phosphonate tenofovir (R)-PMPA on double-stranded DNA Cauliflower mosaic virus (CaMV) in Brassica pekinensis plants grown in vitro on liquid medium was evaluated. Double antibody sandwich ELISA and PCR wereused for relative quantification of viral protein and detecting nucleic acid in plants. (R)-PMPA at concentrations of 25 and 50 mg/l significantly reduced CaMV titers in plants within 6?9 weeks to levels detectable neither by ELISA nor by PCR. Virus-freeplants were obtained after 3-month cultivation of meristem tips on semisolid medium containing 50 mg/l (R)-PMPA and their regeneration to whole plants in the greenhouse. Studying the metabolism of (R)-PMPA in B. pekinensis revealed that mono- and diphosphate, structural analogs of NDP and/or NTP, are the only metabolites formed. The data indicate very low substrate activity of the enzymes toward (R)-PMPA as substrate.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA522%2F09%2F0707" target="_blank" >GA522/09/0707: Effect of acyclic nucleosid phosphonates and other antimetabolites against plant viruses</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Antiviral Research
ISSN
0166-3542
e-ISSN
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Volume of the periodical
92
Issue of the periodical within the volume
2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
4
Pages from-to
378-381
UT code for WoS article
000297427200030
EID of the result in the Scopus database
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