Association of aster yellows subgroup 16SrI-C phytoplasmas with a disease of Ribes rubrum

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00367360" target="_blank" >RIV/60077344:_____/11:00367360 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Association of aster yellows subgroup 16SrI-C phytoplasmas with a disease of Ribes rubrum

Original language description

A Ribes rubrum plant showing malformation and twisting of branches was found in a garden in South Bohemia. Ultrathin sections of tissues from symptomatic shoots revealed the presence of phytoplasma-like bodies. Different primer sets were used for amplification of the 16S-23S ribosomal gene segment. RFLP analysis and sequencing for phytoplasma identification classified the detected phytoplasma in the aster yellows group, subgroup 16SrI-C. Successful transmission of detected phytoplasma by dodder to periwinkle was confirmed by detection of numerous phytoplasma bodies in ultrathin sections of C. roseus and by PCR from dodder and periwinkle. RFLP analyses of PCR products as well as nucleotide sequences of the currant plant and symptomatic periwinkles wereidentical. Sequenced data obtained from both currant and indicator plant, were aligned and sequences of 1,613 bp were found to be identical. Transmissions of phytoplasma by grafting to healthy currant rootstocks were unsuccessful.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/QH91224" target="_blank" >QH91224: Research on methods for pathogen-free propagation material of currants</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bulletin of Insectology

ISSN

1721-8861

e-ISSN

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Volume of the periodical

64

Issue of the periodical within the volume

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Country of publishing house

IT - ITALY

Number of pages

2

Pages from-to

65-66

UT code for WoS article

000296681900030

EID of the result in the Scopus database

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