Phytoplasma: Methods and Protocols. Dodder Transmission of Phytoplasmas

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00425832" target="_blank" >RIV/60077344:_____/13:00425832 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Phytoplasma: Methods and Protocols. Dodder Transmission of Phytoplasmas
Original language description
Transmission of phytoplasmas from naturally infected plant host species using the parasitic plant Cuscuta spp. to Catharanthus roseus is an effective way to maintain a wide range of phytoplasmas for further research. Here, transmission via dodder from aninfected plant Rehmannia glutinosa var. purpurea and from a symptomatic red currant plant (Ribes spp.) to C. roseus indicator plants using a ?stable bridges method is described. Typical symptoms of phytoplasma disease on periwinkle plants were obtained:virescent flowers with an increased number of axillary shoots and smaller leaves on periwinkle plants after transmission from R. purpurea, and greening flower petals (virescence) after transmission from Ribes spp. Phytoplasmas could be detected in donorand recipient plants by electron microscopy and by PCR assays using universal phytoplasma primer pairs. RFLP analyses of PCR fragments can also be used to confirm the identity of the phytoplasmas from donor and recipient plants.
Czech name
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Czech description
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Project
<a href="/en/project/QH91224" target="_blank" >QH91224: Research on methods for pathogen-free propagation material of currants</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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