Ligand-binding properties of a juvenile hormone receptor, Methoprene-tolerant
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00369355" target="_blank" >RIV/60077344:_____/11:00369355 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/11:43882689
Result on the web
<a href="http://dx.doi.org/10.1073/pnas.1116123109" target="_blank" >http://dx.doi.org/10.1073/pnas.1116123109</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1073/pnas.1116123109" target="_blank" >10.1073/pnas.1116123109</a>
Alternative languages
Result language
angličtina
Original language name
Ligand-binding properties of a juvenile hormone receptor, Methoprene-tolerant
Original language description
Juvenile hormone (JH) is a terpenoid, a small lipophilic molecule of vital importance to insects. The molecular action of JH has for long decades remained an enigma, because a JH receptor could not be identified. In this study we provide conclusive evidence for the JH receptor role of the bHLH-PAS domain transcription factor Methoprene-tolerant (Met). By testing Met point mutant proteins in vitro, we determined specific amino acids contributing to the high-affinity JH binding. Using mutants unable to bind JH, we demonstrated that interactions of Met with partner proteins require the ligand-binding capacity of Met. Therefore, Met resembles certain nuclear receptors, e. g., of retinoic acid or thyroid hormone, but since Met belongs to a different proteinfamily, it establishes a prototype of a hormonal receptor of a novel class. Our results address a long-standing question of the mechanism of action of juvenile hormone and its insecticidal analogs.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
e-ISSN
—
Volume of the periodical
108
Issue of the periodical within the volume
52
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
21128-21133
UT code for WoS article
000298479900054
EID of the result in the Scopus database
—