In situ derivatization-liquid liquid extraction as a sample preparation strategy for the determination of urinary biomarker prolyl-4-hydroxyproline by liquid chromatography-tandem mass spectrometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00375767" target="_blank" >RIV/60077344:_____/12:00375767 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/12:43894048 RIV/00843989:_____/12:00102350
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1002/jms.2952/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jms.2952/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jms.2952" target="_blank" >10.1002/jms.2952</a>

Result language
angličtina
Original language name
In situ derivatization-liquid liquid extraction as a sample preparation strategy for the determination of urinary biomarker prolyl-4-hydroxyproline by liquid chromatography-tandem mass spectrometry
Original language description
The derivatization with alkyl chloroformates proceeds smoothly in aqueous media, and the desired reaction products are efficiently separated from interfering ionic components by their extraction into a water-immiscible organic phase. Here, this approachwas examined in detail for analysis of a potential urinary dipeptide biomarker L-prolyl-4-L-hydroxyproline (PHP) by liquid chromatography coupled to electrospray mass spectrometry. PHP was treated with a series of alkyl and fluoroalkyl chloroformates. The N-isobutyloxycarbonyl isobutyl ester derivative afforded a highest detector response from all the derivatized forms examined, including the nonderivatized PHP. The LC-MS/MS method was validated using a heptadeutero-PHP and PHP thus determined in humanurine in less than 5 min. The in-situ derivatization-liquid liquid extraction has been demonstrated to be a useful sample preparation strategy for the analysis of polar metabolites by LC-MS/MS in complex urine matrix.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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