Towards characterization of DNA structure under physiological conditions in vivo at the single-molecule level using single-pair FRET

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00381618" target="_blank" >RIV/60077344:_____/12:00381618 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/12:43883364

Result on the web

<a href="http://dx.doi.org/10.1093/nar/gks333" target="_blank" >http://dx.doi.org/10.1093/nar/gks333</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gks333" target="_blank" >10.1093/nar/gks333</a>

Result language

angličtina

Original language name

Towards characterization of DNA structure under physiological conditions in vivo at the single-molecule level using single-pair FRET

Original language description

Here we explored parameters critical for characterization of NA structure using FRET in the complex cellular environment of a living E. coli cell. Measurements indicated that the uncertainty in determining the orientation factor of fluorophores, which translates into precision of inter-probe distance determination, was the main limiting factor for the quantitative structural analysis of nucleic acids in living cells. Our data suggest that despite its low precision, the in-cell FRET data could be appliedto assess the consistency of structural data acquired from FRET efficiency values measured in a complex cellular environment with various structural models. The main advantage of the in-cell spFRET setup presented here over other established techniquesallowing conformational analysis in vivo is that it allows structural investigation of NA structure in any cell type and, most importantly, in a native cellular environment, which is not disturbed by either introduced bulk NA or by the us

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

<a href="/en/project/KAN200100801" target="_blank" >KAN200100801: Bioactive Biocompatible Surfaces and Novel Nanostructured Composites for Applications to Medicine and Drug Delivery</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

40

Issue of the periodical within the volume

16

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

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UT code for WoS article

000308959800002

EID of the result in the Scopus database

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