In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F09%3A00334099" target="_blank" >RIV/61388955:_____/09:00334099 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/09:00334099

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer

Original language description

Expression of the nascent RNA transcript is regulated by its interaction with a number of proteins. Misregulation of such interactions often results in impaired cellular functions that can lead to cancer and many diseases. Our understanding of RNA?protein interaction within the cellular context is thus essential for development of novel diagnostic and therapeutic tools. While there are many in vitro methods analyzing RNA?protein interactions, in vivo approaches are scarce. Here we established a method based on fluorescence resonance energy transfer (FRET), which we termed RNA-binding mediated FRET (RB-FRET). The methods enables determination of RNA?protein interaction inside cells and was tested on hnRNP H protein binding to its cognate RNA. Using twodifferent approaches, we provided evidence that RB-FRET was sensitive enough to detect specific RNA?protein interactions in cell, providing a powerful tool to study spatial and temporal localization of specific RNA?protein complexes.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

R N A

ISSN

1355-8382

e-ISSN

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Volume of the periodical

15

Issue of the periodical within the volume

11

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

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UT code for WoS article

000270851000014

EID of the result in the Scopus database

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